Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Amphiploid aquatic animal and method of breeding same

An aquatic animal, diploid technology, applied in animal husbandry and other directions to achieve the effect of restoring reproductive capacity

Inactive Publication Date: 2007-08-29
MATSUMOTO INST OF MICROORGANISMS +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the amphiploid produced in the theoretical example shown in the above-mentioned Fig. 13 becomes a X A X B X B X Chromosomally female or with A X A X B Y B Y Chromosomally male, but the amphidiploid produced by this theoretical method has not yet been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Amphiploid aquatic animal and method of breeding same
  • Amphiploid aquatic animal and method of breeding same
  • Amphiploid aquatic animal and method of breeding same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 is a double diploid using a cross between goldfish and koi,

[0061] The inventor crossed the female of the koi carp with the male of the goldfish, and successfully produced a diploid fish with XXXY-type sex chromosomes with reproductive ability.

[0062] background

[0063] Koi has a variety of colors such as gold and silver that cannot be seen in goldfish. Therefore, it is necessary to introduce the color of koi to goldfish. Goldfish and carp have an inter-genus relationship, so hybrids cannot form normal gametes. However, female hybrids sometimes produce non-reduced eggs in which chromosomes are not reduced. On the other hand, male hybrids cannot produce non-reduced sperm, but a large number of hybrid F1 were studied, and it was found that there were hybrid F1 that produced non-reduced sperm.

[0064] The 7 individuals surveyed in the females all produced non-reduced eggs, while the 15 individuals surveyed in the males were all non-reproductive. It is clear tha...

Embodiment 2

[0078] In this example, rainbow trout was crossed with angler salmon to obtain the diploid of the present invention 3.

[0079] The double diploid described in the second aspect of the present invention is a type XXXY produced by preventing the second cleavage of hybrid eggs, temporarily obtaining the first generation of double diploids, and fertilizing the reduced eggs and sperm Double diploid. The inventors discovered that the mechanism of the multiplication treatment considered to be the prevention of the first cleavage in order to produce tetraploid is actually not the case, and it is the prevention of the second cleavage. This is revealed by the following experiment.

[0080] The fertilized eggs of rainbow trout or hybrid eggs of rainbow trout and shovel salmon were cultivated at 10 degrees, and after 5 hours and 15 minutes, treated with 650 atmospheres of water pressure for 6 minutes, and then bred at 10 degrees.

[0081] Immediately before and after the treatment, fix the e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides an amphidiploid aquatic animal with genome AB of different species, which has XXXY type sex chromosome with reproductive ability. Select male non-reduced sperm and female non-reduced eggs from many hybrid first-generation aquatic animals housed in a closed system, and use the non-reduced sperm to fertilize the above-mentioned non-reduced eggs to produce reproductive capacity Amphidiploid aquatic animals with XXXY sex chromosomes. Owing to the XXXY-type sex chromosomes, mating with the egg AXBX of the hybrid F1 that produces non-reduced eggs is possible, and the next generation of amphidiploids can be stably produced through natural mating. During meiosis, since each set of chromosomes of the same kind is provided exactly, the shape and quality of the first generation (F1) can be permanently maintained. Since no genetic segregation occurs, two sets of each must be provided by different species of genome, so even if inbreeding is repeated, the effects of inbreeding depression will not be exhibited.

Description

Technical field [0001] The present invention relates to double diploid aquatic animals such as fish, shellfish and crustaceans, especially the double diploid aquatic animals produced stably without genetic segregation in the next generation of hybrids, and a breeding method thereof. Background technique [0002] In interspecies or intergeneric crosses of aquatic animals such as fish, fertilization occurs and embryos begin to occur. However, the hybrid is almost always fatal. Even if it can survive, the chromosome non-homology causes the synergy insufficiency, does not form gametes, and becomes infertile. Even if the hybrids between closely related species can be reproductive, the next generation of genetic segregation will occur, and the traits (heterosis) superior to the parental generation will decrease with each generation. [0003] Fig. 11 and Fig. 12 schematically show the genome combination in the generation of the hybrid and the first generation of the hybrid. In addition,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A01K67/033
CPCA01K67/02A01K67/027
Inventor 小野里坦
Owner MATSUMOTO INST OF MICROORGANISMS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com