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K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith

A technology of phosphorus-dissolving microorganisms and organic fertilizers, applied in the field of intensive agricultural production, can solve the problems of nutrient resource loss in the soil-plant system, waste of raw materials, and weaken the ability of sustainable agricultural development, etc., to achieve wide sources, increase available phosphorus, Effect of enhancing colonization and ability to function

Active Publication Date: 2007-11-07
PENYAO ENVIRONMENTAL PROTECTION
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] On the other hand, the solid organic waste in my country's agricultural product processing industry and the livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste raw materials that can be used as organic fertilizers and microbial organic fertilizer products; a large amount of The loss of nutrient resources (C, N, P, K, S and trace elements) outside the soil-plant system obviously weakens the ability of my country's agricultural sustainable development

Method used

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  • K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith
  • K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith
  • K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith

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Embodiment Construction

[0026] (1) Obtainment of Phosphorus-Solubilizing Strain

[0027] Select well-growing plant rhizosphere from calcareous phosphorus-deficient soil to collect soil and plant root system, store the samples at low temperature (4-15℃) and bring them back to the laboratory; adopt a series method to use PVK-modified solid plate selective medium (glucose 10g , tricalcium phosphate 5g, ammonium sulfate 0.5g, sodium chloride 0.2g, magnesium sulfate 0.1g, potassium chloride 0.2g, yeast extract 0.5g, manganese sulfate 0.002g, ferrous sulfate 0.002g, 0.4% bromophenol blue 6ml, agar 18g, distilled water 1000ml, pH natural) under the condition of 37.5 ℃ for 36-48 hours to obtain a single colony of phosphate-solubilizing bacteria, select the strain with a larger ratio of phosphate-solubilizing circle to colony circle for further shaker screening; use NBRIP liquid medium (Glucose 10g, Tricalcium Phosphate 5g, Magnesium Chloride 5g, Magnesium Sulfate 0.25g, Potassium Chloride 0.2g, Ammonium Sulf...

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Abstract

The present invention relates to agricultural production technology, and is especially one kind of K3 bacterial strain capable of dissolving calcium phosphate in soil and microbial fertilizer produced with the K3 bacterial strain. The bacterium strain with powerful calcium phosphate dissolving capacity is separated from limy soil, used in liquid fermentation, and mixed with specific organic carrier to produce phosphate decomposing microbial organic fertilizer, which contains phosphate decomposing microbes in 5x10<7> / g, total nitrogen in 4 wt%, total potassium in 1-2 wt%, total phosphorus in 1-2 wt% and organic matter in 35 wt%. Test shows that the phosphate decomposing microbial organic fertilizer can provide phosphorous nutrients in the effect similar to chemical phosphate fertilizer, and the phosphate decomposing bacterium can reach soluble phosphorus concentration in calcium phosphate solution up to 643 mg / l.

Description

1. Technical field [0001] The invention provides a bacterial K3 strain capable of dissolving soil calcium phosphate and a microbial organic fertilizer prepared therefrom, belongs to the technical field of agricultural intensive production, and is specially used for overcoming the problem of crop phosphorus deficiency on calcareous soil. 2. Background technology [0002] After chemical phosphate fertilizers are applied to the soil, the crops in the current season can only use about 15%, and about 85% of the phosphate fertilizers are fixed in the soil and gradually age and lose their effectiveness. . Therefore, farmers have to apply chemical phosphate fertilizers every season. In this way, on the one hand, a large number of phosphate rock resources are depleted. It is predicted that the existing high-grade phosphate rock resources in my country will be completely exhausted in only 30 years at the current development and utilization rate. On the one hand, farmers apply phosphor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C12R1/385
Inventor 沈其荣杨兴明徐阳春黄启为
Owner PENYAO ENVIRONMENTAL PROTECTION
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