K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith
A technology of phosphorus-dissolving microorganisms and organic fertilizers, applied in the field of intensive agricultural production, can solve the problems of nutrient resource loss in the soil-plant system, waste of raw materials, and weaken the ability of sustainable agricultural development, etc., to achieve wide sources, increase available phosphorus, Effect of enhancing colonization and ability to function
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[0026] (1) Obtainment of Phosphorus-Solubilizing Strain
[0027] Select well-growing plant rhizosphere from calcareous phosphorus-deficient soil to collect soil and plant root system, store the samples at low temperature (4-15℃) and bring them back to the laboratory; adopt a series method to use PVK-modified solid plate selective medium (glucose 10g , tricalcium phosphate 5g, ammonium sulfate 0.5g, sodium chloride 0.2g, magnesium sulfate 0.1g, potassium chloride 0.2g, yeast extract 0.5g, manganese sulfate 0.002g, ferrous sulfate 0.002g, 0.4% bromophenol blue 6ml, agar 18g, distilled water 1000ml, pH natural) under the condition of 37.5 ℃ for 36-48 hours to obtain a single colony of phosphate-solubilizing bacteria, select the strain with a larger ratio of phosphate-solubilizing circle to colony circle for further shaker screening; use NBRIP liquid medium (Glucose 10g, Tricalcium Phosphate 5g, Magnesium Chloride 5g, Magnesium Sulfate 0.25g, Potassium Chloride 0.2g, Ammonium Sulf...
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