Production of micro-solid phase extraction chip based on micro-processing method and using method

Abstract

The invention relates to a miniature solid phase extract chip which is manufactured based on the micro processing method and its application method. The chip includes at least a cap rock and a pipeline level, the states pipeline level forms at least a section of solid phases extracts padding packing pipeline and a section through the micro processing filters pipeline, among, the solid phase extract padding packing pipeline through dam type structure or the stockade structure partially separates from the neighboring pipeline, but keeps the partial gas phases and the liquid phase connectivity with the neighboring pipeline, the filter pipeline partially is the pore structure which forms by the light initiation polymer; simultaneously at the back of the pipeline level corresponds the solid phase extract padding packing pipeline region process has the miniature heating electrode and the temperature induction electrode, which is used to heating control temperature. The invention may apply to the analytical chemistry and the biochemistry domain micro sample fast extract.

Description

technical field [0001] The present invention relates to a micro solid phase extraction chip and its use method, more precisely to a micro solid phase extraction chip and its use method based on the micro processing method, the chip can be applied to the fields of analytical chemistry and biological analysis and detection . Background technique [0002] Solid-phase extraction (Solid-Phase Extraction, SPE) is currently one of the most popular sample pretreatment techniques. It is based on the traditional liquid-liquid extraction (LLE), combined with the similar solubility mechanism of substance interaction and the basic knowledge of the stationary phase in HPLC and GC, which are widely used at present, and gradually developed. It is mainly used for sample extraction. Isolation, purification and concentration. Compared with liquid-liquid extraction (LLE), SPE has the advantages of simple and fast operation, less organic solvent consumption, and less environmental pollution. ...

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Problems solved by technology

[0003] Conventional SPE devices are usually composed of SPE small columns and accessories, often involving column tubes, sintered pads, fillers, vacuum systems, vacuum pumps, drying devices, inert gas sources, large-capacity samplers and buffer bottles, etc., which are difficult to implement Miniaturization and integration, and not suitable for the processing of micro samples, thus limiting the automation and portability of the entire analysis process

Although the solid phase microextraction (Solid Phase Microextraction, SPME) technology developed in the 1990s has made good improvements in the miniaturization of the device and the ease of operation, it still has great limitations in terms of integration. Difficult to integrate with front-end sample rough processing and back-end detection

Although the existing microfluidic SPE chip has successfully realized the combination of solid phase extraction technology and microfluidic chip, it has not done enough in terms of integration with the front and rear ends, which limits the promotion and use of this new technology.

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