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Method for preparation of collagen / chitose supporting stand of composite hydroxylapatite

A technology of compounding hydroxyapatite and chitosan, which can be used in medical science, prosthesis and other directions, can solve the problem of difficult to obtain cell active sites, and achieve the effect of good application prospects, promotion of migration, and rich material sources.

Active Publication Date: 2010-12-08
无锡贝迪生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional bone repair materials are difficult to obtain cell active sites to promote the expression of normal activity of osteoblasts

Method used

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  • Method for preparation of collagen / chitose supporting stand of composite hydroxylapatite
  • Method for preparation of collagen / chitose supporting stand of composite hydroxylapatite
  • Method for preparation of collagen / chitose supporting stand of composite hydroxylapatite

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] 1) Collagen / chitosan is dissolved in the acetic acid solution that mass concentration is 3%, and the mass ratio of collagen and chitosan is 5: 5, and the collagen / chitosan solution that obtains mass concentration is 0.5%, injects into mold , freeze at -20°C for 2 hours, freeze at -20°C, freeze-dry in a lyophilizer for 24 hours, take it out from the mold, and then soak it in an acetic acid solution with a mass concentration of 0.3%. Fully washed with distilled water, placed in 0.25% aqueous glutaraldehyde solution for cross-linking at 4°C for 12 hours, and fully washed with triple distilled water to obtain a cross-linked collagen / chitosan scaffold. figure 1 is the scanning electron micrograph of the cross-linked collagen / chitosan scaffold.

[0024] 2) Prepare 0.05mol / L CaCl 2 Aqueous solution and 0.03mol / L diammonium hydrogen phosphate solution; Step 1) gained cross-linked collagen / chitosan scaffold in 0.05mol / L CaCl 2 Take it out after soaking in the aqueous solution ...

example 2

[0028] Step 1) is the same as step 1) of Example 1, but the mass ratio of collagen and chitosan is 9: 1;

[0029] Step 2) with the step 2 of example 1), prepared the collagen / chitosan support of depositing hydroxyapatite, but CaCl 2 The concentration of the aqueous solution is 0.1mol / L, and the concentration of the corresponding diammonium hydrogen phosphate solution is 0.06mol / L;

[0030] Step 3) is the same as step 3) of Example 1, but the mineralization time is 12 hours; Figure 6 is a scanning electron micrograph of hydroxyapatite particles within a collagen / chitosan scaffold of mineralized composite hydroxyapatite.

example 3

[0032] Step 1) is the same as step 1) of Example 1, but the mass ratio of collagen / chitosan is 8: 2;

[0033] Step 2)~3) with the step 2)~3) of example 1; Figure 7 It is the change of the water absorption rate of the sedimentation scaffold and the mineralization scaffold obtained under different calcium chloride solution infiltration conditions.

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Abstract

The present invention discloses preparation process of collagen / chitosan rack with compounded hydroxyapatite. The preparation process includes the steps of: preparing cross-linked collagen / chitosan rack, chemical depositing to compound hydroxyapatite onto the rack, and mineralizing to obtain active compound bone rack. The preparation process is simple and has abundant material source and high production efficiency. The collagen / chitosan rack in bone-like structure has introduced active hydroxyapatite, and possesses the advantages of high biocompatibility, excellent comprehensive performance, use convenience and other advantages. The collagen / chitosan rack with compounded hydroxyapatite can promote the migration, growth and differentiation of bone cell, is favorable to raising the efficiency of repairing damaged bone tissue, and possesses excellent application foreground.

Description

technical field [0001] The invention relates to a preparation method of a collagen / chitosan scaffold compounded with hydroxyapatite. Specifically, through the bionic simulation of human bone structure and function, collagen / chitosan and hydroxyapatite are used to simulate the extracellular matrix of bone cells, providing a method for preparing composite structural scaffolds for bone repair. Background technique [0002] Bone injury is a common disease nowadays. Many patients suffer from articular bone injuries caused by various bone and joint diseases such as rheumatism and rheumatoid or sports trauma. So far, clinically, there is still a lack of effective methods for repairing large-sized bone defects. Applying the methods and principles of regenerative medicine to repair bone tissue is an important means at present, and has achieved good results. Among them, bone repair scaffolds play a very important role in bone regeneration. [0003] Bone is a general term for a gro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/44
Inventor 高长有马列赵海光沈家骢
Owner 无锡贝迪生物工程股份有限公司
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