Breeding method of oil-bearing crop with high content of carotene

A carotene and oil crop technology, applied in the fields of botanical equipment and methods, analytical materials, organic chemistry, etc., can solve problems such as failure to propose research purposes and solve problems, and achieve high economic value, accurate results, and high reliability. Effect

Inactive Publication Date: 2011-10-05
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

At home, the inventor of this patent has established a rapid measurement technology of rapeseed carotenoid content (Gao Guizhen, etc., research on the method for measuring the total amount of rapeseed carotenoids, Journal of Plant Genetics, 2005, 6: 414-417), Started the screening of rape variety resources, but did not put forward a clear research purpose and a technical solution to the problem

Method used

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  • Breeding method of oil-bearing crop with high content of carotene
  • Breeding method of oil-bearing crop with high content of carotene
  • Breeding method of oil-bearing crop with high content of carotene

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Embodiment Construction

[0035] The method of the present invention is: 1) use spectrophotometry to screen a large number of germplasm resources, or use genetic engineering technology to overexpress key genes for carotenoid synthesis to obtain seeds with a carotenoid content higher than 60 μg·gFW -1 2) Using the basic germplasm with high carotenoids as the donor and the fine variety as the recipient, use conventional breeding techniques to carry out hybridization and transfection to obtain high-carotenoid high-quality varieties; 3) Use HPLC method to determine the selection and breeding The content of carotenoids in the seeds of varieties should be controlled at 60-300μg·gFW -1 within range.

[0036]Oil crops are rapeseed, soybean, peanut, sesame, sunflower, safflower, flax and corn.

[0037] In order to understand the present invention better, take rape as an example below, further set forth content of the present invention:

[0038] refer to figure 1 , the concrete steps of the present invention ...

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Abstract

The present invention provides a cultivation method of oil plant containing rich vitamin A and relates to the oil plant breeding technology. The method is that the spectrophotometer is used for selecting large amounts of seed resource or the gene engineering technology is used for over-expressing the crucial genes of synthesizing the carotenoid; the basic germ plasm of seeds selected contain the carotenoid which is higher than 60Mug.gFW<-1>; the basic germ plasm with high carotenoid content is taken as the donor and the high-quality seeds are taken as the acceptor; the conventional breeding technology is used for hybridizing to obtain high-quality breeds with high carotenoid; the HPLC method is used for detecting the carotenoid content of the breeding seeds to control the content in the range of 60-300Mug.gFW<-1>. The selecting method of the present invention is simple with high efficiency, accurate results, low cost and reliable resources. The plant oil squeezed from the oil plant cultivated with the method of the present invention contains rich vitamin A and is characterized by high nutrition and anti-aging, disease resistant and deterioration resistant functions.

Description

technical field [0001] The invention relates to the technical field of breeding of new varieties of oil crops. Background technique [0002] Carotenoids are a class of pigments derived from isoprenoids that are widely found in plants and other organisms. In plants, carotenoids are an important part of the photosynthetic system, assisting light energy absorption and having antioxidant effects. At the same time, carotenoids are also essential for human nutrition and health. Existing studies have shown that carotenoids can have the following functions: ①Carotenoids, especially β-carotene, are the precursors of vitamin A (Provitamin A, provitamin A), and vitamin A is an essential micronutrient for the human body ; ②It has immune function, which can improve the ability of the human immune system to resist pathogens; ③It has the function of antioxidant, which can quench the adverse effects of singlet oxygen and remove free radicals in the body, thereby delaying human aging; ④It ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/63A01H1/02C07C403/24G01N21/00C12N15/82
Inventor 伍晓明高桂珍陆光远陈碧云许鲲李响枝
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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