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Animal source pancreas obtaining method for preparing pancreatic island transplant

An acquisition method and animal-derived technology, applied in medical science, surgery, etc., can solve the problems of affecting the number and activity of islets, affecting the effect of islet transplantation, and achieve the goal of shortening ischemia time, reducing ischemic damage, and improving islet yield. Effect

Inactive Publication Date: 2008-03-12
王维
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AI Technical Summary

Problems solved by technology

The rationality of the process of obtaining islets directly affects the quantity and activity of obtained islets, which ultimately affects the effect of islet transplantation

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Using fresh live newborn pigs less than 5 days old as a donor, the steps to obtain pancreas are:

[0020] (1) After the donor animal is anesthetized (unconscious), first wash it with detergent-containing water once, and then wash it twice with 84 disinfectant, the detergent is a commercial household detergent;

[0021] (2) Fix the above-mentioned donor animal in a supine position in a sterile tray, spray complexed iodine solution (other similar surgical disinfectants can also be sprayed) on the operation area of ​​the donor animal, and place the tray in a sterile operating table;

[0022] (3) Aseptic operation, the surgeon and assistant first cut the abdominal skin and all layers of the abdominal wall structure other than the parietal peritoneum in the middle of the upper abdomen (under the xiphoid process) of the donor animal, enter the chest cavity, and cut open The heart was bleeding to death.

[0023] (4) Open the abdominal cavity of the donor animal; use hemostatic for...

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Abstract

An animal source pancreas acquisition method used for preparing transplantation islets is provided. The steps include that a donor animal which is anesthetized is cleaned with water containing detergent for one time at first and cleaned with disinfection solution for two times. A supine position of the donor animal is fixed on an aseptic pallet. Complex iodine solution is sprayed on an operation area of the donor animal. And the pallet is arranged on an aseptic operation platform. An aseptic operation is started. An operator and an assistant firstly incise the abdominal skin in the middle of the upper abdomen of the donor animal in a plus shape and every layer of an abdominal wall structure except a wall peritoneal layer. The peritoneal of the donor animal is opened in the same plus shaped way. The operator incises a diaphragm upwards into a pleural of the animal. A heart is sheared open for bloodletting until the animal is dead. A homeostatic forceps is used to clamp a stomach. Then, the stomach is turned up. And an islet is exposed out. The islet is gently and finely separated and fetched out. The islet is fetched out and is arranged in cold Hanks liquid for preparation. The process should be implemented under a low temperature environment below 4 CEG C. The method can effectively improve the acquisition rate of the islets, protect the activity of the islets and supply usable sources for the preparation of the transplantation of the islets.

Description

Technical field [0001] The invention relates to a method for obtaining animal-derived pancreas for preparing transplanted islets. Background technique [0002] Nowadays, insulin injections are often used in clinical treatment of diabetes. Although external insulin can control the changes of patients' blood sugar to a certain extent, it cannot well control and prevent complications, such as the occurrence and development of diabetic nephropathy and diabetic retinopathy, while diabetic complications are It is the main cause of death of diabetic patients. [0003] Xenogeneic islet transplantation is a promising method for the treatment of diabetes. Obtaining sufficient transplantable islets from xenogeneic donors (such as newborn pigs) is the basic prerequisite for this method. The rationality of the process of obtaining islets directly affects the number and activity of the obtained islets, which ultimately affects the effect of islet transplantation. Therefore, it is very importan...

Claims

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Application Information

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IPC IPC(8): A61B17/00
Inventor 王维叶斌
Owner 王维
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