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Method for exactly extracting antiviral field pennycress polysaccharide from field pennycress

A technology of precise extraction and Patrinia, which is applied in the direction of antiviral agents, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problems of anti-virus and ineffectiveness, and achieve the effect of high safety

Inactive Publication Date: 2010-06-09
李鑫 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem that the Patrinia polysaccharides extracted in the prior art contain protein components and nucleic acids that are ineffective against viruses, the present invention provides a method for accurately extracting antiviral Patrinia polysaccharides from Patrinia

Method used

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  • Method for exactly extracting antiviral field pennycress polysaccharide from field pennycress
  • Method for exactly extracting antiviral field pennycress polysaccharide from field pennycress

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specific Embodiment approach 1

[0006] Specific embodiment 1: The method for accurately extracting antiviral sagebrush polysaccharides from sagebrush of this embodiment is carried out in the following steps: 1. The crude extract eluates extracted from sagechis spp. are dissolved in distilled water to a concentration The eluate solution is 80~120mg / mL; 2. Add chloroform with the volume of the eluate solution 1 / 6~1 / 4 and n-butanol with the volume of the eluate solution 1 / 18~1 / 12, shake 15~ After 30 minutes, centrifuge the mixed solution at a speed of 3500-4000r / min for 12-18 minutes to remove the denatured protein and lower turbid liquid at the junction; 3. Repeat the operation of step 2 for the upper aqueous solution 3-7 times to obtain a deproteinized solution 4. Add 5 times the volume of the deproteinized solution and 95% mass concentration of ethanol for precipitation, and then stand for 8 to 14 hours to separate the precipitate and supernatant; 5. Take the precipitate and dissolve it with distilled water an...

specific Embodiment approach 2

[0009] Embodiment 2: The difference between this embodiment and Embodiment 1 is: in step 1, the crude extract eluate extracted from Patrinia vulgaris is dissolved in distilled water into an eluate solution with a concentration of 90-110 mg / mL . The other steps and parameters are the same as in the first embodiment.

specific Embodiment approach 3

[0010] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is: in step 1, the crude extract eluate extracted from the Paeonia solani is dissolved in distilled water into an eluate solution with a concentration of 100 mg / mL. The other steps and parameters are the same as in the first embodiment.

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Abstract

The invention discloses relates to a method for exactly extracting antiviral herba patriniae polysaccharide from herba patriniae, and relates to a method for extracting the antiviral herba patriniae polysaccharide. The invention solves the problem that the herba patriniae polysaccharide extracted by the prior art includes protein component and nucleic acid which are ineffective to prevent viruses.The method for exactly extracting the effectively the antiviral herba patriniae polysaccharide from the herba patriniae comprises the following steps: step one, solution preparation; step two, protein removal; step three, the repetition of the step two; step four, ethyl alcohol adding anddeposition; step five, chromatography and filter; namely, the effectively antiviral herba patriniae polysaccharide in the herba patriniae is attained. The effectively antiviral herba patriniae polysaccharide extracted by the invention does not include the protein component and the nucleic acid which are ineffective to prevent the viruses, the antiviral effect is obvious, and the security is high.

Description

Technical field [0001] The present invention relates to a method for extracting antiviral Paitinensis polysaccharide. Background technique [0002] The patent No. ZL03100182.3 published in China on July 28, 2004, "Method for Extraction of Anti-Respiratory Syncytial Virus Substances from Patrinia vulgaris" is able to obtain Patrinia vulgaris against RSV. However, it is a crude extract containing protein components and nucleic acids that are not effective against viruses, which is not conducive to the pharmacodynamics research of Paisinica sp. polysaccharide and the clinical application of drugs. Summary of the invention [0003] The present invention provides a method for accurately extracting anti-virus polysaccharides from Patrinia vulgaris in order to solve the problem that the polysaccharides from Patrinia vulgaris extracted by the prior art contain ineffective protein components and nucleic acids against viruses. [0004] The method of the present invention for accurately extra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P31/12
Inventor 李鑫李洪源
Owner 李鑫