Method and device for accomplishing dark-field photomicrography and fluorescent photomicrography by axicon lens

Abstract

The invention relates to a method and a device for achieving dark-field microscopy and fluorescent microscopy by using cone mirror. The method comprises the following steps of: first generating a parallel illumination light beam or a fluorescence-induced light beam; diverging the parallel illumination light beam by the cone mirror to form a hollow beam; transmitting the hollow beam to sequentially pass through a lens I and a lens II to make the divergence angle of the emergent light from the lens II larger than an aperture angle of a microscopic objective lens; and disposing a sample at the center of the hollow beam and observing the sample by the microscopic objective lens, wherein a CCD camera can be arranged in front of the microscopic objective lens. The inventive system has high transmissivity, can conveniently achieve switching between bright-field microscopy and dark-field microscopy and can simultaneously achieve dark-field microscopy and fluorescent microscopy functions, thus solving the technical problems of prior dark-field microscopy with low illumination light transmissivity and the technical problems of prior fluorescent microscopy which needs different filters for different fluorescent dyes and is incompatible to dark-field microscopy.

Description

technical field [0001] The invention relates to a method and device for realizing dark field microscopy and fluorescence microscopy. Background technique [0002] Optical microscope is an important tool for studying the microscopic world. According to different observation methods and observation objects, optical microscopes can be divided into bright field microscopes, dark field microscopes, and fluorescence microscopes. Most of the research objects can be observed by bright field microscope, but some samples are difficult to observe by using ordinary bright field microscopy because their refractive index is very close to the surrounding environment. In this case, dark field microscopy or fluorescence microscopy is used can solve this problem. The principle of dark field microscopy is to block the middle part of the illumination source, and only let the light at the edge of the illumination beam obliquely illuminate the sample at a certain angle. In this way, the illumin...

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Problems solved by technology

[0005] The object of the present invention is to propose a method and device capable of realizing dark-field microscopy and fluorescence microscopy, using an axicon and a group of lenses to match as a concentrating device, It can realize dark-field microscopy, fluorescence microscopy and bright-field microscopy at the same time, which solves the problem that the illumination light transmittance of the existing dark-field microscopy is not high and the existing fluorescence microscopy needs to use different filters for different fluorescent dyes And cannot have the technical problem of dark field microscopy at the same time

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