Regulation and control of lincomycin course of fermentation by using ammonium ion and improvement of lincomycin volume of production

Abstract

The invention pertains to the field of microbial fermentation and discloses an optimized lincomycin fermentation production method. The method includes that, the ammonium ion concentration in an initial fermentation culture medium is regulated to 57 plus or minus 5 mmol / L for culturing the streptomyces lincolnensis; when the ammonium ion concentration in the fermentation culture medium is 0.2 to 1.5 mmol / L, the ammonium ions are supplemented, then the ammonium ion concentration in the fermentation culture medium is controlled within 2 to 5mmol / L till the end of the fermentation. The invention studies the impact factors for producing lincomycin by utilizing the streptomyces lincolnensis on the large-scale level and sums up the optimized lincomycin fermentation method for the first time; the yield of the lincomycin can be greatly improved by using the method.

Description

technical field

[0001] The invention belongs to the field of microbial fermentation, and more specifically, the invention relates to an optimized lincomycin fermentation production method. Background technique

[0002] Lincomycin (Lincomycin) belongs to the lincosamine antibiotics. The fermentation process of Streptomyces lincolnensis (S.lincoinensis) to produce lincomycin is very complicated, and its biosynthetic route is still unclear. way.

[0003] At present, there are only studies on the factors affecting the fermentation process of lincomycin on the shake flask scale, but due to the limitations of mixing and sampling, shake flask fermentation can neither fully develop the production potential of Streptomyces lincomycin, nor can it feed back the fermentation in time Dynamic adjustment information of the process. However, there is no report on the research on the factors affecting the fermentation of lincomycin in a large-scale bioreactor. Contents of the invention ...

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