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Phomopsis rhzomorph compound and its preparation method and application

A technology of Phomopsis and compounds, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problem of less symbiotic fungi in seaweed

Inactive Publication Date: 2008-08-06
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few domestic studies on seaweed symbiotic fungi, and so far there are few research reports on the active substances of seaweed fungi

Method used

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  • Phomopsis rhzomorph compound and its preparation method and application
  • Phomopsis rhzomorph compound and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A. Medium formula: slant medium formula: take 200g potatoes, peeled, cut into small pieces, add water to boil for 30min, filter with 4 layers of gauze, add 20g glucose, 20g agar to the filtrate, use 50% seawater (seawater and tap water by volume) than mixing) to 1000mL. Seed culture medium formula, add 20g of glucose and 20g of agar to each liter of potato filtrate (preparation method is the same as above), and use 50% seawater (mixture of seawater and tap water by volume) to make up to 1000mL. Fermentation medium formula: same as seed medium.

[0039] B. Fermentation process: slant culture: prepare culture medium according to the above slant culture medium formula, divide into test tubes, sterilize at 121° C. for 20 minutes, and prepare slant culture medium. Pick the Phomopsis sp. hzla01-1 (Phomopsis sp.hzla01-1) and transfer it to the slant medium, cultivate at 28°C for 3 days; prepare the medium according to the formula of the seed medium, sterilize (121°C, 30min), ...

Embodiment 2

[0044] A. Medium formula: slant medium formula: take 300g potatoes, peeled, cut into small pieces, add water to boil for 30min, filter with 4 layers of gauze, add 30g glucose, 30g agar to the filtrate, use 100% seawater (seawater and tap water by volume) than mixing) to 1000mL. Seed culture medium formula, add 30g of glucose and 30g of agar to each liter of potato filtrate (preparation method is the same as above), and use 100% seawater (mixed with tap water by volume) to make the volume to 1000mL. Fermentation medium formula: same as seed medium.

[0045] B. Fermentation process: slant culture: prepare culture medium according to the above slant culture medium formula, divide into test tubes, sterilize at 121° C. for 20 minutes, and prepare slant culture medium. Pick the Phomopsis sp. hzla01-1 (Phomopsis sp.hzla01-1) and transfer it to the slant medium, and cultivate it at 28°C for 5 days; prepare the medium according to the formula of the seed medium, sterilize (121°C, 30mi...

Embodiment 3

[0050] A. Medium formula: slant medium formula: take 150g potatoes, peeled, cut into small pieces, add water to boil for 30min, filter with 4 layers of gauze, add 15g glucose, 15g agar to the filtrate, use 30% seawater (seawater and tap water by volume) than mixing) to 1000mL. Seed medium formula, add 15g glucose and 15g agar to each liter of potato filtrate (preparation method is the same as above), and dilute to 1000mL with 30% seawater (mixed by volume of seawater and tap water). Fermentation medium formula: same as seed medium.

[0051] B. Fermentation process: slant culture: prepare culture medium according to the above slant culture medium formula, divide into test tubes, sterilize at 121° C. for 20 minutes, and prepare slant culture medium. Pick the Phomopsis hzla01-1, homopsis sp.hzla01-1) and transfer it to the slant medium, cultivate at 28°C for 7 days; prepare the medium according to the seed medium formula, sterilize (121°C, 30min), and put the seeds The medium i...

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Abstract

A phomopsis compound, a relative preparation method and an application relate two compounds. The invention provides a phomopsis compound of sea weed fungus with strong antibacterial activity and application in antibacterial drugs, a relative preparation method and an application, wherein the sea weed fungus is hzla01-1(Phomopsis sp.hzla01-1) as phomopsis compound A and B, compound A is colorless oily substance, and the compound B is colorless acicular crystal. The preparation method comprises seed cultivation of phomopsis hzla01-1, fermentation of phomopsis hzla01-1, extraction of phomopsis compound and refining phomopsis compound. Pharmacological verification proves that the compound has significant antibacterial function and can be used to prepare new antibacterial drug.

Description

technical field [0001] The present invention relates to two kinds of compounds, in particular to a class of phosphamin compounds with antibacterial activity derived from seaweed fungi - Phomopsis hzla01-1 (Phomopsissp.hzla01-1) and preparation methods thereof, and their preparation method Application in the preparation of antibacterial drugs. Background technique [0002] The clinical application of antibacterial drugs has brought about fundamental changes in the occurrence and transition of infectious diseases, and also brought two consequences, namely, changes in common clinical pathogens and changes in bacterial resistance. Therefore, the search for efficient, broad-spectrum and safe antibacterial drugs is a very urgent problem. [0003] Due to its special living environment (high salt, high pressure, low temperature, low light and oligotrophy), marine microorganisms can synthesize some antibacterial drugs with novel structures, which is one of the important resource lib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61K31/336A61P31/04C12P17/08C12R1/645
Inventor 郑忠辉杜希萍沈月毛黄耀坚宋思扬苏文金
Owner XIAMEN UNIV
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