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Porcine circovirus 2 type inactivated vaccine

A technology of porcine circovirus and circovirus, applied in antiviral agents, virus/bacteriophage, virus antigen components, etc., can solve the problems of virus isolation, cultivation and identification, and achieve simple process, good antigenicity, High safety effect

Inactive Publication Date: 2008-08-13
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the virus is difficult to isolate, identify, and cultivate. Therefore, there is no effective vaccine for the prevention and treatment of the disease in my country.

Method used

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  • Porcine circovirus 2 type inactivated vaccine
  • Porcine circovirus 2 type inactivated vaccine
  • Porcine circovirus 2 type inactivated vaccine

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Experimental program
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Embodiment Construction

[0019] 1. Sources and standards of poisonous seeds

[0020] The strain used to manufacture and test this product is the PCV2-SH strain, which was isolated and identified from a pig farm in Shanghai by the Key Open Laboratory of Animal Disease Diagnosis and Immunity of the Ministry of Agriculture of Nanjing Agricultural University in 2002, named Shanghai isolate ( referred to as SH strain).

[0021] 1.1 Poison Seed Standard

[0022] Virus content: The virus was serially diluted 10 times with PRM-1640 maintenance solution, and 10 -3 、10 -4 、10 -5 3 dilutions, each dilution was inoculated into 4 wells of a 96-well culture plate PK-15 cell monolayer, 0.2ml per well, and a negative control was set up at the same time, and continued to culture for 48 hours in an incubator containing 5% CO2 at 37°C. Fix the cells with absolute ethanol, measure the number of wells containing PCV2 positive cells (brownish yellow) in each dilution with the immunoperoxidase monolayer assay (IPMA), c...

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Abstract

The pig circular ring virus 2 type (PVC2) inactivated vaccine (SH individual plant) of the invention belongs to biotechnology field. The pig circular ring virus 2 type poisonous individual plant SH belongs to circular ring virus section circular ring virus genus which has been preserved in Wuhan institute of virology, Chinese academy of sciences. The shanghai separated individual plant SH of purified PCV2 virus is obtained by gathering raw material from hogpen which happened bad weaning piglet multisystem exhaustion failure syndrome in Shanghai in 2002 year, separating, appraising and purifying virus. The PCV2-SH plant is proliferated in mass in PK-15 cell, inactivated through methyl aldehyde and emulsified with liquid paraffine adjuvant to prepare conventional liquid paraffin(e) adjuvant immunomodulators for vaccines. The laboratory has trial-manufactured five lots vaccines successfully which are good safety and also can induce pig bring immune protection effect, made out a draft rules for vaccines production and testing. The inactivated vaccine proved by every aspects experiment has met state biological products standard completely.

Description

1. Technical field [0001] The porcine circovirus type 2 inactivated vaccine of the invention belongs to the high-tech field of biotechnology, and is specially used for enhancing the immune protection effect of pigs against PCV2 and reducing the economic loss of pig farms. 2. Technical background [0002] PCV-2 is a swine disease worldwide, which has caused huge losses to the swine industry in the world. The virus mainly causes multisystemic wasting syndrome and nephrotic dermatitis syndrome in weaned piglets. At the same time, the disease can also cause immunosuppression to a certain extent, and it is easy to cause secondary or concurrent infection of other diseases, especially the disease and PRRS have mutual synergy. Because the virus is a DNA virus, the mutation rate of the virus is low, and at the same time, only PCV2 is the cause of disease worldwide. At the same time, the virus itself has good antigenicity, which can induce the body to produce high levels of antibodi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N7/02A61K39/12A61P31/20C12R1/93
Inventor 姜平王先炜李玉峰
Owner NANJING AGRICULTURAL UNIVERSITY
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