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Method for evaluating or screening hair growth-regulating agent

A technology for hair growth regulators and growth promoters, which is applied in the field of evaluation or screening of hair growth regulators, and can solve the problems of unsuitable mass screening, difficulty in obtaining human hair follicles, unsuitable screening, etc.

Inactive Publication Date: 2013-03-06
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Screening using organ culture of human hair follicles has high data reliability, but human hair follicles themselves are not easy to obtain, so it is not suitable for mass screening
In addition, screening by organ culture of hair follicles of mice and rats is often available, but it is not suitable for large-scale screening from the viewpoint of animal protection because experimental animals are used.

Method used

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  • Method for evaluating or screening hair growth-regulating agent
  • Method for evaluating or screening hair growth-regulating agent
  • Method for evaluating or screening hair growth-regulating agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Separation of hair follicles

[0053] As the pig skin, use the skin obtained from a white-haired hybrid pig raised in Japan as a meat pig, cut it to an appropriate size, cut off excess fat, and put it in chlorhexidine under a sterile environment. solution (diluted 5% chlorhexidine solution (manufactured by Sumitomo Pharmaceutical Co., Ltd.) with water to 5 to 20 times), soaked for 5 to 10 minutes, disinfected, and then washed several times with D-PBS.

[0054] Next, under a solid microscope, hair follicles were separated from the treated pig skin with tweezers and a scalpel (FEATHER No 10), and concentrated in the culture medium. As the culture medium, RPMI1640 medium (Gibco cat. No. 11835-030), William's Medium E medium (Gibco cat. No. 1255 1-032) and DMEM / HamF12 (1:1) medium (Gibco cat. No. 11039-021).

[0055] (2) Effects of DHT on pig hair follicle organ culture

[0056] Pig hair follicles were obtained from the pig's back, flanks, abdomen, groin, buttocks, a...

Embodiment 2

[0068] Evaluation of embodiment 2 hair growth regulator

[0069] (1) t-flavanone and TGF-β2

[0070] Pig buttock hair follicles were obtained in the same manner as in Example 1. As a medium, RPMI1640 (Gibco cat. No. 11835-030) medium was used. A medium in which a penicillin / streptomycin (Gibco cat. No. 15140-122) solution was added at a ratio of 1% to RPMI1640 medium was used.

[0071] Using the above-mentioned RPMI1640 medium, t-flavanone (trans-3) which was confirmed to have a hair growth-promoting effect in organ culture of beard hair follicles of rats as an active ingredient of a hair growth-promoting agent not belonging to pharmaceuticals was prepared. , 4′-dimethyl-3-hydroxyflavanone (Hotta et al., Fragrance Journal, Vol. 31 No. 2, 33-40 (2003))), the final concentration in the culture medium 1 μM (for the synthesis method, refer to Japanese Patent Laid-Open No. 2000-198779). Furthermore, TGF-β2 (R&D System, Inc., model number: 102-B2-001 / CF), which was confirmed to ...

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Abstract

The present invention provides a method for evaluating or screening a hair growth-regulating agent which utilizes a readily available animal. The present invention is directed to a method for evaluating or screening a hair growth-regulating agent which comprises subjecting hair follicles of a pig to an organ culture in the presence of a test substance, and evaluating or selecting a substance which promotes or suppresses the growth of the hair follicles.

Description

technical field [0001] The present invention relates to methods for evaluating or screening hair growth regulators using pig hair follicles. Background technique [0002] Generally, with regard to hair, it is often desired to promote hair growth or suppress hair growth depending on the site. For example, many people suffer from hair thinning and hair loss due to stress, genetics, etc. In addition, some people feel troublesome about the so-called useless hair disposal. [0003] Regarding hair, it is known that there are androgen-dependent hairs and androgen-independent hairs. For example, it is considered that hair loss from the forehead to the top of the head or male pattern baldness in which the part recedes, and hairs such as beard, leg hair, and armpit hair are closely related to androgen, while hair on the temporal region, hair on the back of the head, Eyebrows and eyelashes are less affected by androgen (Non-Patent Document 1, Non-Patent Document 2). [0004] Theref...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/15G01N33/48
Inventor 笹嶋美知代伊藤纪子森脇繁布施千绘
Owner KAO CORP