Fast reproducing method for high quality seedling of dendrobium officinale

Abstract

The invention provides a method for rapidly reproducing high quality germchit with dendrobium officinale, which is characterized in that: (1) folded sprouts of the dendrobium officinale are taken andsterilized, and stem apexes are inoculated to a solid culture medium to establish a non-bacterial system; (2) the stem sections or small basal tissue blocks of the young seedlings of the non-bacterialsystem are cut for inducing protocorm based on suitable hormone combination and a culture medium; (3) the protocorm is cultured alternatively on liquid and solid subculture mediums for rapid multiplication to form protocorm masses; (4) the protocorm masses are cut apart and transferred on a solid planting medium for disuniting plants into small seedlings; (5) the small seedlings are transferred on a solid strong seedling culture medium for strengthening the seedlings and taking roots to culture complete plants; (6) the non-bacterial strong seedlings are fixedly planted in suitable seedling adapting substrate to obtain the high quality germchit. The method can be used in large scale industrialization and the explants have the advantages of high soil removal efficiency, high multiplicationrate, low variability, strong germchit, good quality, high survival rate after being transplanted, strong growth potential, etc.

Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to a rapid propagation method for high-quality dendrobium officinale seedlings. Background technique

[0002] Dendrobium (Dendrobium) is a large genus of Orchidaceae (Orchidaceae), including more than 1,600 plants. There are about 63 species in my country, of which more than 30 are used for medicine, mainly including Dendrobium (D. Dendrobium (D.fimbriatum), Dendrobium officinale (D.candidum), Dendrobium officinale (D.chrysanthum) and Dendrobium nobile (D.nobile), among which Dendrobium officinale is the most precious. Traditional Chinese medicine believes that Dendrobium has the functions of nourishing yin and detoxifying, promoting body fluid and quenching thirst, improving eyesight and strengthening body. Modern medical research has found that Dendrobium contains a variety of alkaloids, polysaccharides and amino acids, which have many functions of enhancing immune function,...