Fast reproducing method for high quality seedling of dendrobium officinale

Abstract

The invention provides a method for rapidly reproducing high quality germchit with dendrobium officinale, which is characterized in that: (1) folded sprouts of the dendrobium officinale are taken andsterilized, and stem apexes are inoculated to a solid culture medium to establish a non-bacterial system; (2) the stem sections or small basal tissue blocks of the young seedlings of the non-bacterialsystem are cut for inducing protocorm based on suitable hormone combination and a culture medium; (3) the protocorm is cultured alternatively on liquid and solid subculture mediums for rapid multiplication to form protocorm masses; (4) the protocorm masses are cut apart and transferred on a solid planting medium for disuniting plants into small seedlings; (5) the small seedlings are transferred on a solid strong seedling culture medium for strengthening the seedlings and taking roots to culture complete plants; (6) the non-bacterial strong seedlings are fixedly planted in suitable seedling adapting substrate to obtain the high quality germchit. The method can be used in large scale industrialization and the explants have the advantages of high soil removal efficiency, high multiplicationrate, low variability, strong germchit, good quality, high survival rate after being transplanted, strong growth potential, etc.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a rapid propagation method for high-quality dendrobium officinale seedlings. Background technique [0002] Dendrobium (Dendrobium) is a large genus of Orchidaceae (Orchidaceae), including more than 1,600 plants. There are about 63 species in my country, of which more than 30 are used for medicine, mainly including Dendrobium (D. Dendrobium (D.fimbriatum), Dendrobium officinale (D.candidum), Dendrobium officinale (D.chrysanthum) and Dendrobium nobile (D.nobile), among which Dendrobium officinale is the most precious. Traditional Chinese medicine believes that Dendrobium has the functions of nourishing yin and detoxifying, promoting body fluid and quenching thirst, improving eyesight and strengthening body. Modern medical research has found that Dendrobium contains a variety of alkaloids, polysaccharides and amino acids, which have many functions of enhancing immune function,...

AI Technical Summary

Problems solved by technology

However, the natural reproductive rate of wild Dendrobium is low, the growth is slow, and the harvest yield is low, which is far from meeting the needs of the market. In addition, people's long-term unrestrained and indiscriminate digging have caused serious damage to wild resources.

Although there are researches on Dendrobium plant tissue culture and artificial cultivation Dendrobium technology at present, there are problems such as long seedling production cycle, high production cost, low reproduction coefficient, uneven quality, etc., and are satisfied with large-scale industrial production. Seedling rapid propagation method is not reported

[0003] At present, the explants of Dendrobium officinale tissue culture technology mostly use the fern fruit seeds of wild plants, which are sterilized and inoculated onto the induction medium to form protocorms to establish an aseptic system. Problems such as limitations, inconsistent traits, rapid degradation of seedling quality, serious aging after several subcultures, and difficulty in preserving high-quality germplasm