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Preparation method of chronic liver injury unit stress depression rat model

A technology of chronic liver injury and rat model, applied in preparations for in vivo experiments, pharmaceutical formulations, etc., can solve problems such as no clear understanding of the mechanism

Inactive Publication Date: 2008-10-15
刘铁军
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AI Technical Summary

Problems solved by technology

The occurrence of hepatitis combined with depression has been reported at home and abroad, but there is no clear understanding of its mechanism

Method used

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  • Preparation method of chronic liver injury unit stress depression rat model
  • Preparation method of chronic liver injury unit stress depression rat model
  • Preparation method of chronic liver injury unit stress depression rat model

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Experimental program
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Embodiment Construction

[0066] Select male Wistar rats with a body weight of 200g, and after acclimatization to the environment for 1 week, carry out behavioral screening of the open box experiment, and select rats with similar scores;

[0067] 1-4 weeks, 5 animals / cage, normal diet and water, 40% CCL 4 1ml / kg, subcutaneous injection 2 times / week;

[0068] Week 5-12, 5 animals / cage, normal diet and water, 40% CCL 4 1ml / kg, subcutaneous injection 2 times / week, intragastric administration of normal saline, 1 time / day;

[0069] In the 13th week, 5 animals / cage, normal diet and drinking water, intragastric administration of normal saline, 1 time / day;

[0070] In the 14th to 16th week, the open-box experiment was carried out first, 1 cage / cage, with normal diet and drinking water, except for the stimulation of fasting and water deprivation, intragastric administration of normal saline, 1 time / day, and chronic stress stimulation, randomly selected 1 rat per day. kind;

[0071] On the 112th day, body ...

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Abstract

A preparation method of chronic hepatic injury complicated with stress depression rat model comprises selecting male Wistar rat (body weight of 180-220 g), acclimating for one week, performing open field test ethology screening, and selecting rat having close integration; normally drinking and eating for the first week to the fourth week, and performing hypodermic injection of 40% CCL4 1ml / kg (twice per week); normally drinking and eating from the fifth to the twelfth week, performing hypodermic injection of 40% CCL4 1ml / kg (twice per week), and performing gastric perfusion of normal saline (one time per day); normally drinking and eating in the thirteenth week, and performing gastric perfusion of normal saline (one time per day); performing open field test in the fourteenth to sixteenth week (one rat per cage), normally drinking and eating, performing gastric perfusion of normal saline (one time per day), and offering chronic stress stimulation; and weighing in 112th day, and performing sweet water consumption and open field test. The preparation method provides reference for modeling research of chronic hepatic injury complicated with stress depression, provides effective experiment base for clinical diagnosis of hepatopathy complicated with depression, and provides experimental verification means for the research of drug for treating the hepatopathy complicated with depression.

Description

technical field [0001] The invention relates to a method for preparing an animal experiment model, in particular to a method for preparing a rat model of chronic liver injury combined with stress and depression. Background technique [0002] Chronic liver injury, namely chronic hepatitis, mainly refers to chronic hepatitis B and chronic hepatitis C, and my country is one of the countries with high incidence. Because of its characteristics of infectivity, refractory, malignant transformation, family heredity, and frequent physical discomfort, patients with chronic hepatitis suffer from both mental and physical torture for a long time or intermittently. Therefore, patients with chronic hepatitis are in a state of depression for a long time. They are pessimistic and disappointed in life and lose confidence in survival. We call this depressed state caused by chronic hepatitis "chronic hepatitis combined with depression". In addition to the common symptoms and signs of chronic ...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 刘铁军
Owner 刘铁军
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