Process for separating total alkaloids form Chinese medicament extracting solution

Abstract

The invention discloses a method for separating total alkaloid of traditional Chinese medicines, in particular a method for separating the total alkaloid from the extraction solution of traditional Chinese medicines and belongs to the field of traditional Chinese medicines. The method includes the technical proposal as follows: the extraction solution of traditional Chinese medicines is taken; the pH value is adjusted between 1 and 7; the solution is filtered; the filtrate is added to a cation exchange resin column; firstly, water is used for elution and impurity removal; then the resin column is immersed with 0.5 to 20 percent of the salt solution; 0.5 to 20 percent of the salt solution with acid or ethanol is used for elution until no alkaloid is tested; and the eluate is collected; thealkali is added for neutralization and then the total alkaloid of traditional Chinese medicines is obtained after the concentration drying through the desalted treatment. The method overcomes the defects of the low extraction rate, a large amount of organic solvents, too high acid base concentration, complex process, no mass production, etc., in the prior art and can separate the highly purified total alkaloid from the extraction solution of traditional Chinese medicines with high efficiency.

Description

technical field [0001] The invention relates to a method for extracting and separating effective components of traditional Chinese medicines, in particular to a method for extracting and separating total alkaloids from extracts of traditional Chinese medicines, and belongs to the field of traditional Chinese medicines. Background technique [0002] Traditional Chinese medicine alkaloids are a class of important substances in nature, and more than 700 kinds of them have been isolated at present. They are the largest type of active ingredients in traditional Chinese medicine with definite curative effect and wide application, especially for cancer, liver disease, cardiovascular and cerebrovascular diseases, and rheumatism. It has unique curative effects, such as berberine in Coptis chinensis for antibacterial and anti-inflammatory, ephedrine in ephedra for asthma, reserpine in Rauwolfia for blood pressure, and galantamine in Lycoris for children. Paralysis sequelae have curati...

AI Technical Summary

Problems solved by technology

But for the refinement of alkaloids, the biggest deficiency of this method is: firstly, it is difficult to adsorb, and the sample liquid must be a dilute solution, and the alkalinity should be suitable: the alkaloids are still in an ionized state when the alkalinity is low, and the resin The adsorption rate is low; the alkalinity is high, the alkaloids are free, and the macromolecular alkaloids are poorly soluble and precipitated, but still cannot be loaded on the column

However, the steps of the above-mentioned method are cumbersome, and it is difficult to realize industrially processes such as organic solvent extraction or organic solvent reflux extraction of the resin, and the efficiency of eluting alkaloids with lye is very low

At present, this method only stays at the level of preparing small samples in the laboratory, and cannot achieve large-scale production.

[0009] In summary, the above methods generally have defects such as high cost, large consumption of organic solvents, poor selectivity, low purity of total alkaloids, and inability to realize large-scale production. Therefore, purification technology is still the "bottleneck" for the separation of total alkaloids from traditional Chinese medicine. question

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