Uses of BMP-7 in preparing medicament for preventing and/or treating liver fibrosis
A technology of BMP-7 and liver fibrosis, which is applied in the direction of drug combination, drug delivery, non-active ingredients of polymer compounds, etc.
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[0032] 1. Successfully prepared rat liver fibrosis model:
[0033] The immune rat model of liver fibrosis was induced by repeated intraperitoneal injection of pig serum. Pig serum 0.5ml / time, intraperitoneal injection to experimental rats, twice a week, a total of 8 weeks. Such as figure 1 shown.
[0034]2. Successfully isolated and cultured hepatocytes and hepatic stellate cells from model animals.
[0035] Hepatic cells and hepatic stellate cells of experimental animals were separated by double enzyme (type I collagenase and pronase E) portal perfusion and shaving centrifugation. Such as figure 2 shown.
[0036] 3. To observe the effects of BMP-7 on collagen production and secretion of primary hepatocytes respectively.
[0037] BMP-7 in the present invention is purchased from Sigma Company in the United States, and the purity is greater than 95% (the BMP-7 freeze-dried powder of each package contains 0.2 μm BMP-7, 33% cyanogen, 0.1% trifluoroacetic acid, 0.5 mg bovine...
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