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Method for determining amino acid concentration and amino acid diagnosis/determination reagent kit

A technology for concentration determination and amino acid, applied in the field of amino acid diagnosis/determination kits and determination of amino acid concentration, can solve the problems of poor specificity, high cost of equipment and complicated operation methods, etc.

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing determination methods include methods such as chromatography, electrochemical method, instrumental analysis method (amino acid analyzer), spectrophotometer, etc., and these methods have disadvantages such as comparatively complicated operation methods, poor specificity, or high cost of instruments and equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] The amino acid diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0094] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0095] Stabilizer 500mmol / L

[0096] Amino acid oxidase 10000U / L

[0097] Peroxidase 30000U / L

[0098] 4-amino antiarsenic 2mmol / L

[0099] Carbolic acid 10mmol / L

[0100] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0101] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the amino acid sample to be tested is 1 / 25, and the reaction direction is positive reaction (rising) reaction), the detection method is the endpoint / rate method, the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0102] After adding samples and rea...

Embodiment 2

[0104] The amino acid diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0105] Reagent 1

[0106] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0107] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0108] Reagent 2

[0109] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0110] Stabilizer 500mmol / L

[0111] Amino acid oxidase 10000U / L

[0112] Peroxidase 30000U / L

[0113] 4-amino antiarsenic 0.6mmol / L

[0114] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0115] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 546nm, test sub-wavelength 600nm, the volume ratio of the amino acid sample to be tested is 1 / 20, and the reaction direction is positive reaction (rising) reaction), the detection method is the endpoint / rate method, the del...

Embodiment 3

[0118] The amino acid diagnosis / measurement reagents of this embodiment are three reagents, including:

[0119] Reagent 1

[0120] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0121] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0122] Reagent 2

[0123] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0124] Dimethylaniline 2mmol / L

[0125] Reagent 3

[0126] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0127] Stabilizer 500mmol / L

[0128] Amino acid oxidase 10000U / L

[0129] Peroxidase 30000U / L

[0130] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0131] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 578nm, test sub-wavelength 660nm, the volume ratio of the amino acid sample to be tested is 1 / 30, and the reaction direction is positive reacti...

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PUM

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Abstract

The invention relates to a kit for diagnosing / mensurating amino acid by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the amino acid, and belongs to the technology field of medical / food / industrial / agricultural / environmental inspection and measurement. The main components of the kit include a buffer solution, amino acid oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the amino acid directly.

Description

technical field [0001] The invention relates to an amino acid diagnosis / measurement kit, and at the same time, the invention also relates to a method for determining amino acid concentration, which belongs to the technical field of medicine / food / industry / agriculture / environmental inspection and determination. Background technique [0002] The determination of amino acid content is an important determination item in medicine / food / industry / agriculture / environment. Existing determination methods include chromatography, electrochemical method, instrumental analysis method (amino acid analyzer), spectrophotometer and other methods. These methods have disadvantages such as relatively complicated operation methods, poor specificity, or high equipment costs. [0003] Amino acid is not a simple substance, 17 kinds of amino acids can be directly measured with an amino acid analyzer (the instrument is expensive and cannot be widely used). For medicine / food / industry / agriculture / environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD