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Method for extracting francisella native from bykomycin fermentation liquor

A technology of framectin sulfate and neomycin sulfate, which is applied in the direction of fermentation, antibacterial drugs, amino sugar, etc., can solve the problems of high organic solvent residue, large consumption of raw materials, and low analysis yield, so as to improve product quality , Low production cost, and the effect of reducing environmental pollution

Inactive Publication Date: 2010-12-08
SHIJIAZHUANG HUASHU PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many shortcomings in the above method, for example, the resin type used in the past has small adsorption capacity, low analysis yield, large consumption of raw materials, and high cost
Methanol is used in the crystallization process, which is costly, pollutes the environment, and has high residues of organic solvents in the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Adsorb the neomycin sulfate fermentation liquid with a strong acidic cationic resin, rinse with water to remove impurities, analyze with 0.2M ammonia water, concentrate and decolorize the analysis solution, and the total unit of the neomycin concentrate prepared is 190,000-200,000 units / milliliter, adjust the pH value to 5-9, add neomycin sulfate concentrated solution to the mass volume ratio of resin to be 10% for each column, add to the top of the weakly acidic cationic resin treatment column, open the outlet valve, and control the flow rate to about 50ml / min, after the neomycin sulfate concentrated solution is adsorbed by a weakly acidic cation, the neomycin sulfate C is eluted with 0.1M ammonia solution to remove impurities. The flow rate is controlled at 1 / 500-1 / 300 resin volume per minute. After the impurities have been eluted, continue to elute neomycin sulfate C with 0.1M ammonia solution, and monitor the optical rotation of the solution at any time. When the...

Embodiment 2

[0025] Adsorb the neomycin sulfate fermentation broth with a strong acidic cationic resin, rinse with water to remove impurities, analyze with 0.2M ammonia water, concentrate the analysis solution, and prepare a neomycin concentrate with a total unit of 190,000-200,000 units / ml. Adjust the pH value to 5-9, add neomycin sulfate concentrated solution to the mass volume ratio of resin to each column to be 10%, add to the top of the weakly acidic cationic resin treatment column, open the outlet valve, control the flow rate to about 50ml / min, After the neomycin concentrate is adsorbed by weakly acidic cations, it is eluted with 0.5M ammonia solution to remove impurities. The flow rate is controlled at 1 / 500-1 / 300 resin volume per minute. After the impurities have been eluted, continue to elute neomycin C with 0.5M ammonia solution, and monitor the optical rotation of the solution at any time. When the optical rotation appears at the highest point and begins to decline, monitor with...

Embodiment 3

[0028] Adsorb the neomycin fermentation broth with a strong acidic cationic resin, rinse with water to remove impurities, analyze it with 0.2M ammonia water, concentrate and decolorize the solution, and prepare the neomycin concentrate with a total unit of 190,000-200,000 units / ml , adjust the pH value to 5-9, add neomycin sulfate concentrate to the mass volume ratio of resin to 10% of each column, use a pump to hit the top of the weakly acidic cationic resin treatment column, open the outlet valve, and control the flow rate to about 50ml / min, after the neomycin concentrate is adsorbed by weakly acidic cations, it is eluted with 0.8M ammonia solution to remove impurities, and the flow rate is controlled at 1 / 500-1 / 300 resin volume per minute. After the impurities have been eluted, continue to elute neomycin sulfate C with 0.5M ammonia solution, and monitor the optical rotation of the solution at any time. When the optical rotation appears the highest point and begins to declin...

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PUM

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Abstract

The invention relates to a preparation method of a medicine raw material, in particular to a method for extracting sulfuric acid fra avermectin from fermentation broth of neomycin sulfate. The preparation method is as follows: fermentation broth of neomycin sulfate is adsorbed by strong acidic ion exchange resin, parsed, concentrated and decolorized; the concentrated and decolorized concentrated solution of the neomycin sulfate is adsorbed by weak acidic cation exchange resin; ammonia of 0.1 M to 1.0 M is used to elute the neomycin sulfate B in the saturated resin in the first procedure; and eluent is concentrated, acidified, decolorized, spray dried and micronized, and sulfuric acid fra avermectin is prepared. The preparation method solves the problems existing in the prior art that the analytical yield is low, the raw materials are consumed a lot, the cost is high as the methanol is used in the crystallization process, the methanol pollutes the environment, and a lot of organic solvent remains in the product. The preparation method has the advantages that the adsorption selectivity of the resin is improved, the used amount of the eluent is reduced, no organic solvent remains in the product, the environmental pollution is reduced, the yield is improved, the quality of the product is improved, and the production cost is low.

Description

technical field [0001] The invention relates to a method for preparing pharmaceutical raw materials, in particular to a method for extracting framectin sulfate from neomycin sulfate fermentation liquid. Background technique [0002] Framectin sulfate (neomycin sulfate B) is an aminoglycoside antibiotic isolated from neomycin sulfate. Enterobacteriaceae such as Klebsiella and Proteus also have good effects, overcome the side effects of neomycin sulfate, and can be made into injections, powders, and ointments. The commonly used preparation method of framectin sulfate is to separate it from neomycin sulfate fermentation broth. The neomycin sulfate fermentation liquid is composed of neomycin sulfate B, neomycin sulfate C and some impurities, wherein neomycin sulfate B and neomycin sulfate C are stereoisomers each other, and neomycin sulfate B The content of neomycin sulfate is 75-82%; the content of neomycin sulfate C is 15-18%, and the other parts are impurities. The antibac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H5/06A61P31/04C12P19/28
Inventor 李瑞民陈学军赵亮闫贵霞杨彦芬
Owner SHIJIAZHUANG HUASHU PHARMA