Method for preparing high-purity anthocyanin from huckleberry crude extract by adopting absorption resin method

Abstract

The invention discloses a method for preparing high-purity anthocyanin from a huckleberry crude extract by adopting an absorption resin method. The method comprises the following steps: (1) dissolving the huckleberry crude extract in ethanol aqueous solution to serve as a column solution; (2) adsorbing the column solution in a glass column filled with a macroporous resin; (3) receiving the effluent when the colored tape is transferred to the bottom end of the column; and (4) performing rotary evaporation and spray drying treatment on the effluent, thereby obtaining the high-purity anthocyanin. The method disclosed by the invention has the advantages that according to the preparation method, the desorption step in the traditional adsorption and separation process is not needed; because the operation is simple and convenient and the resin can be repeatedly used, the production cost is obviously reduced, the prepared anthocyanin is stable in quality and good in color, the purity of the anthocyanin is higher than 80 percent, the recovery rate of the anthocyanin can be 80-95 percent, and the method is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the separation and purification of anthocyanins in crude bilberry extract, in particular to a method for preparing high-purity anthocyanins from crude bilberry extract by using an adsorption resin method. Background technique [0002] Vaccinium ( Vaccinium spp.) is an evergreen shrub genus under the Rhododendron family (Ericaceae), mostly growing in acidic soils, commonly found in heather thickets, swamps and acidic woodlands. Among them, the most important two kinds of lingonberries are European lingonberries ( Vaccinium myrtillus L. , also known as bilberry) and blueberry (blueberry). Vaccinium berries are rich in anthocyanins, so they have become one of the most popular raw materials for scholars to study natural anthocyanins (Xu Lu, Zheng Jianxian, Research Overview of European Vaccinium Anthocyanins [J], China Food Additives, 2005, 4:43-46). [0003] Anthocyanins are a general term for a class of substances formed by...

AI Technical Summary

Problems solved by technology

[0013] At present, the process of preparing high-purity anthocyanins often requires other technical assistance, such as subcritical extraction, nanofiltration and other means, resulting in low purification efficiency, cumbersome purification steps and difficulty in continuous large-scale production, a significant increase in purification costs, and the yield Low, difficult to large-scale industrial production (Wen Cheng, Jiang Yuanyong, Zhu Anghui, etc., a method for extracting anthocyanins from European lingonberry: China, CN103467545A, 2013-12-25; Fang Hua, Huang Huaxue, A method for extracting anthocyanins from lingonberry Method for extracting anthocyanins: China, CN104098633A, 2014-10-15)