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Antiviral protein and uses thereof

A protein and antiviral technology, applied in the field of proteins, can solve the problems of inability to encode viral proteins, truncated non-functional products, high frequency mutation of G→A, etc., to reduce immunogenicity, facilitate purification or detection, and improve membrane penetration. The effect of efficiency

Inactive Publication Date: 2009-01-14
SHANTOU UNIV MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] or Truncated nonfunctional product due to premature formation of the stop codon
[0013] In addition, APOBEC has a large molecular weight and strong immunogenicity, and direct application to the human body may induce high antibody levels and reduce its antiviral effect

Method used

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  • Antiviral protein and uses thereof
  • Antiviral protein and uses thereof
  • Antiviral protein and uses thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The preparation of embodiment 1 antiviral protein

[0040] The amino acid sequence of the antiviral protein of the present embodiment is: MetGlySerSer HisHisHisHisHisHis SerSerGlyLeuValProArgGlySerHisMetAlaSerMetThrGlyGlyGlnGlnMetGlyArgGlySer GlyGlyGlyGlyGlySerValAspLeuGlu HisHisHisHisHisHis

[0041]This antiviral protein contains a membrane-penetrating peptide domain (GlyArgLysLysArgArgGlnArgArgArgProProGln) and three cytosine deaminase domains derived from the APOBEC family (these three cytosine deaminase domains are sequentially derived from the cytosine at the N-terminal of the hAPOBEC3G molecule 脱氨酶结构域HisProGluMetArgPhePheHisTrpPheSerLysTrpArgLysLeuHisArgAspGlnGluTyrGluValThrTrpTyrIleSerTrpSerProCysThrLysCys、来自hAPOBEC3G分子C端的胞嘧啶脱氨酶结构域HisAlaGluLeuCysPheLeuAspValIleProPheTrpLysLeuAspLeuAspGlnAspTyrArgValThrCysPheThrSerTrpSerProCysPheSerCys、来自hAPOBEC3F分子C端的胞嘧啶脱氨酶结构域HisAlaGluArgCysPheLeuSerTrpPheCysAspAspIleLeuSerProAsnThrAsnTyrGluValThrTrpTyrThrSerTrpSerProCysP...

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Abstract

The invention relates to anti-viral protein. The anti-viral protein is characterized in that the anti-viral protein is fusion protein and comprises a transmembrane peptide structural domain and a tandem body which is formed through at least two cytosine deaminase structural domains originated from an APOBEC family, a transmembrane peptide structural domain and various cytosine deaminase structural domains. The transmembrane peptide structural domain in the anti-viral protein provided by the invention ensures the anti-viral protein to have the transmembrane capability and to freely shuttle among cells and enters the cells in a non-receptor and non-energy dependent way; the cytosine deaminase structural domains originated from the APOBEC family ensures that the anti-viral protein can effectively inhibit the reproduction of human immunodeficiency virus and / or hepatitis B virus after entering cells; because the non-essential sequences in the APOBEC family protein molecules are removed, not only the antiviral activity of the APOBEC family protein molecules is preserved, but also the immunogenicity of the anti-viral protein is reduced, and the transmembrane efficiency of the anti-viral protein is enhanced.

Description

technical field [0001] The present invention relates to protein, in particular, relates to an antiviral protein for treating human immunodeficiency virus (HIV, human immunodeficiency virus) and / or hepatitis B virus (HBV, hepatitis B virus) infection, and this antiviral protein Applications of viral proteins. Background technique [0002] APOBEC family (apolipoprotein B mRNA editing enzyme-catalytic polypeptide family, apolipoprotein B mRNA editing enzyme catalytic polypeptide family) is a newly discovered protein molecule with cytosine deaminase activity in recent years, which can convert cytosine deamination into Uracil (C→U), its family members include: hAPOBEC1 (i.e. human APOBEC1), hAPOBEC2 (i.e. human APOBEC2), hAPOBEC3A (i.e. human APOBEC3A), hAPOBEC3B (i.e. human APOBEC3B), hAPOBEC3C (i.e. human APOBEC3C), hAPOBEC3D ( Human APOBEC3D), hAPOBEC3E (human APOBEC3E), hAPOBEC3F (human APOBEC3F), hAPOBEC3G (human APOBEC3G), hAPOBEC3H (human APOBEC3H), hAPOBEC4 (human APOBEC...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00A61K38/17A61P31/18A61P31/20
Inventor 李卫中李康生王革非辛岗苏芸陈小璇陈幼莹张衡张丹桂曾俊
Owner SHANTOU UNIV MEDICAL COLLEGE
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