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Water-soluble yeast beta-dextran and preparation thereof

A glucan, water-soluble technology, applied in the field of preparation of water-soluble yeast beta-glucan

Active Publication Date: 2010-12-08
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the solubility of degraded oligodextran or modified dextran is improved, the cost of preparation is greatly increased, which also limits a large number of applications in certain fields

Method used

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  • Water-soluble yeast beta-dextran and preparation thereof
  • Water-soluble yeast beta-dextran and preparation thereof
  • Water-soluble yeast beta-dextran and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Take 200g of yeast powder, add 2L of purified water, extract at 80°C for 1h, centrifuge at 4000r / min for 10min, collect the precipitate I, concentrate the supernatant, precipitate with alcohol, and dry to obtain yeast mannan; then add 2L of 4 % (w / v) sodium hydroxide solution, stirred at 60° C. for 1 h, centrifuged at 5000 r / min for 20 min, and collected alkali extraction precipitate III. The supernatant was neutralized to pH 6.0 with 30% citric acid. After cooling to room temperature, it was centrifuged at 5000r / min for 10min. The supernatant was concentrated to 1 / 5 of the volume of the original supernatant, and 95% ethanol was added to make the alcohol concentration in the alcohol solution reach 80%, alcohol precipitation, precipitation and drying to obtain water-soluble yeast β-glucan, the β-glucan content of the product is 85%, and the yield is 15%. Add 2L of water to Alkaline Extraction and Precipitation III, adjust the pH to 8.0, add 2g of alkaline protease, enzym...

Embodiment 2

[0045] Add 2L of purified water to 200g of yeast powder, extract at 100°C for 1h, centrifuge at 4000r / min for 10min, collect the precipitate I, concentrate the supernatant, precipitate with alcohol, and dry to obtain yeast mannan; then add 2L of 10% Sodium hydroxide, stirred at 80°C for 1 h, centrifuged at 3000 r / min for 20 min, and collected the alkali extraction precipitate III. The supernatant was neutralized to pH 7.0 with 10% (w / v) hydrochloric acid. After being cooled to room temperature, it was centrifuged at 3000r / min for 15 minutes. The concentration reaches 80%, alcohol precipitation, precipitation and drying to obtain water-soluble yeast β-glucan, the β-glucan content of the product is 90%, and the yield is 12%. Add 2L of water to Alkaline Extraction Precipitation III, adjust the pH to 7.0, add 1g of papain, enzymatically hydrolyze at 50°C for 16h, collect the precipitate, and dry to obtain 26g of non-water-soluble yeast β-glucan with a content of 95.1%.

[0046] R...

Embodiment 3

[0048] Add 1.2L of purified water to 100g of yeast powder, extract at 121°C for 1.5h, centrifuge at 4000r / min for 10min, collect the precipitate I, concentrate the supernatant, precipitate with alcohol, and dry to obtain yeast mannan; then add 2L of 6 % sodium carbonate solution, stirred and reacted at 60°C for 2h, centrifuged at 12000r / min for 8min, and collected the alkali extraction precipitate III. The supernatant was neutralized to pH 6.0 with 10% (w / v) acetic acid. After cooling to room temperature, it was centrifuged at 12,000 r / min for 5 minutes. After reaching 80%, the alcohol is precipitated, and the water-soluble yeast β-glucan is obtained after the precipitation is dried. The β-glucan content of the product is 85%, and the yield is 10%. Add 1.2 L of water to the alkali extraction precipitation III, adjust the pH to 8.0, add 2 g of alkaline protease, and perform enzymatic hydrolysis at 60°C for 16 hours, collect the precipitate, and dry it to obtain non-water-solubl...

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Abstract

The invention relates to a water-soluble barm Beta-dextran and a preparation method thereof. In the water-soluble barm Beta-dextran chain, Beta-1, 3-dextran is taken as a main chain, Beta-1, 6-dextran is taken as a branched chain, the molecular weight is 0.08 to 0.2 million Daltun. The preparation method of the water-soluble barm Beta-dextran includes the following steps of: water extraction, alkali extraction and impurity processing. The preparation technique of the invention is simple; the technique parameters are easy to be operated and controlled; both the yield and the purity of the product water-soluble barm Beta-dextran are higher. The obtained water-soluble barm Beta-dextran has remarkable immune activation effect and especially can improve the phagocytic function and the antibodyformation capacity of a macrophage to a large extent, thereby improving the body immunity and resistance. The water-soluble barm Beta-dextran can be widely applied in the fields such as food, dairy food and drinks, etc.

Description

Technical field: [0001] The invention relates to a method for preparing zymosan from yeast, in particular to a method for preparing water-soluble yeast beta-glucan. Background technique: [0002] Yeast is a very important industrial microorganism. Most yeasts are processed cheaply as roughage without further processing, and the real comprehensive utilization of by-products cannot be formed. Some enterprises even discharge it directly, causing a great waste of resources and environmental pollution. [0003] β-1,3-glucan is ubiquitous in the cell walls of various fungi, plants and seaweeds. It is a highly biologically active immune polysaccharide. All have good therapeutic effects. β-1,3-glucan is also a good biological effect regulator (BRMs), which can improve the body's immunity by stimulating the immune system. BRMs are very important for maintaining homeostasis in interdependent body systems. Crucially, it helps the body adapt to environmental and psychological stress....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02C12P19/08A61P37/04A23L1/29A23L1/30A23L2/52A23C9/152A23L33/145
Inventor 徐泽平杨传伦丁艳张靖苏亚平王秀芝甄春峰
Owner CHAMBROAD CHEM IND RES INST CO LTD
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