Freeze drying protective agent for rubricyte and use method in freeze drying process

Abstract

The invention relates to a rythrocyte lyophilized protective agent and a using method thereof during lyophilizing. The prescription of the protective agent before being lyophilized is as follows: glucose solution, adenine, NaCl, mannitol, KCl, polymer hydroxyethyl starch, bovine serum albumin and sodium citrate. The prescription of the lyophilized rehydration protective agents is as follows: trehalose, ascorbic acid, NaCl, KH2PO4, Na2 HPO4, dextran, bovine serum albumin, KCl, inosine and adenine. The protective liquid before being lyophilized and rythrocyte suspension liquid are mixed according to the volume ratio of 4:1, are frozen and dried to obtain lyophilized rythrocyte, then the lyophilized rythrocyte is rehydrated by the rehydration protective agent. The recovery rate of the rythrocyte lyophilized protective agent and the using method thereof during lyophilizing of the invention can reach as high as 71.1 percent through the measurement of a cell counter, and is improved by 12.3 percent by comparing with the lyophilized protective agent and the lyophilized method thereof in the prior art, the highest recovery rate of which is 58.5 percent.

Description

technical field [0001] The invention relates to a freeze-drying protective agent for red blood cells and its use method in the freeze-drying process. Before freeze-drying red blood cells, add the freeze-drying protective agent to the red blood cells to be freeze-dried, and then freeze-dry them according to a certain freeze-drying procedure. Recovery rate of red blood cells after lyophilization. Background technique [0002] At present, most of the blood is stored in a refrigerator at 4°C, and the storage period is only about 20 days, which cannot meet the needs of long-term and large-scale storage. It is wasted in vain, but on the other hand, when a large amount of blood is needed when a major disaster (such as an earthquake, war) occurs, the supply is insufficient. Therefore, exploring the long-term preservation technology of red blood cells is a way to alleviate and solve the increasingly prominent clinical blood contradiction. one. [0003] Freeze-drying technology is ...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of high recovery rate of red blood cell rehydration after lyophilization, the present invention proposes a lyoprotectant for erythrocytes and its use method in the process of lyophilization of erythrocytes

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