Artificial culture method for zinc-rich Chinese caterpillar fungus fruiting body and culture medium thereof

A culture method and culture medium technology, applied in the field of pharmacognosy, can solve the problem that there is no corresponding report on the zinc-rich Cordyceps militaris culture method, and achieve significant immune regulation, increase efficacy, and reduce stimulation effects

Inactive Publication Date: 2009-02-25
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no corresponding report on the zinc-rich Cordyceps m

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Strain preparation:

[0035] Cryopreservation of Cordyceps militaris strains: Cordyceps militaris strains 5.701, from China Ordinary Microbial Cultures Preservation Management Center.

[0036] Cordyceps militaris liquid strain: cultured according to the following process steps:

[0037] (1) Bacterial culture (activation) on the slant of the test tube:

[0038] Preparation medium: glucose 10g, peptone 10g, yeast extract 10g, agar powder 10g, water 960g, the pH value is 5.0, and they are divided into test tubes; Cultivate in the dark at ~30°C for 5-10 days.

[0039] (2) Erlenmeyer flask liquid seed culture:

[0040] Prepare medium: glucose 10g, peptone 10g, yeast extract 10g, water 970g, the pH value is 5.0, divide the prepared medium into 500ml Erlenmeyer flasks, each bottle contains 200ml, and seal it at 120-130℃ Sterilize under conditions for 18-30 minutes, take out the medium, and cool naturally to 20-30°C; add 10ml-20ml of cultured test tube liquid strains to...

Embodiment 2

[0054] (1) Strain preparation:

[0055] Cordyceps militaris strains: Cordyceps militaris 5.701, sourced from China General Microorganisms Culture Collection and Management Center.

[0056] (2) Zinc-rich medium preparation:

[0057] Its formula components are: rice 65g, yeast extract 2.0g, water 85g, zinc sulfate 1g, natural pH value;

[0058] (3) Subpackage and sterilize the culture medium:

[0059] Divide the prepared medium into 500ml canned bottles, seal with a special sealing film for edible fungi, and sterilize at 120-130°C for 18-30 minutes;

[0060] (4) cooling, inoculation:

[0061] After sterilization, take out the culture medium, cool it naturally to 20°C-30°C, and insert the solid-cultured Cordyceps militaris under sterile conditions;

[0062] (5) Training:

[0063] Put the inoculated zinc-rich Cordyceps militaris culture medium in a constant temperature incubator, and cultivate it at 20-30°C for 8-12 days. Cultivate for 50-90 days under the condition of -13 h...

Embodiment 3

[0069] (1) Strain preparation:

[0070] Cordyceps militaris strains: Cordyceps militaris 5.701, sourced from China General Microorganisms Culture Collection and Management Center.

[0071] Cordyceps militaris liquid strain: cultured according to the following process steps:

[0072] (1) Bacterial culture (activation) on the slant of the test tube:

[0073] Preparation medium: glucose 20g, peptone 6g, yeast extract 9g, agar powder 15g, water 950g, the pH value is 6.0, and they are divided into test tubes; Cultivate in the dark at ~30°C for 5-10 days.

[0074] (2) Erlenmeyer flask liquid seed culture:

[0075] Prepare medium: glucose 20g, peptone 6g, yeast extract 9g, water 965g, its pH value is 6.0, divide the prepared medium into 500ml Erlenmeyer flasks, each bottle contains 200ml, seal and store at 120-130℃ Sterilize under conditions for 18-30 minutes, take out the medium, and cool naturally to 20-30°C; add 10ml-20ml of cultured test tube liquid strains to each bottle und...

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PUM

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Abstract

The invention discloses an artificial culture method of a zinc-rich cordyceps militaris fruit body and a culture medium thereof. The culture medium comprises the following components by weight portions: 40-65 portions of rice, 0.5-2.0 portions of a yeast extract, 60-85 portions of water and 0.01-1.0 portion of zinc sulfate with the natural PH value. The artificial culture method comprises the following steps: preparation of the culture medium, canning and sterilization; cooling and inoculation; culture; collection of the fruit body; drying and granulation. The method has the advantages of simple operation, short culture period, high yield of the fruit body, no pollution, no waste and zinc supplement function, and the method is suitable for industrialized production.

Description

Technical field: [0001] The invention relates to a method for artificially cultivating fruit bodies of zinc-rich Cordyceps militaris and a culture medium thereof, belonging to the technical field of pharmacognosy. Background technique: [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the Ascomycota subphylum Sclerotinia Sclerotiae Sclerotiae Ergotaceae Cordyceps genus, is one of the precious Chinese medicinal materials in my country. Modern pharmaceutical research has shown that Cordyceps militaris contains nucleosides, polysaccharides, cordycepin, sterols and other components, which are basically the same as Cordyceps sinensis. It has therapeutic effects on diseases such as the immune system, nervous system and cardiovascular system, and has anti-tumor and It has anti-cancer effect and is an ideal substitute of Cordyceps sinensis, which has broad development prospects. [0003] Zinc is a kind of trace element, its content and daily intake in the ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 程显好盖宇鹏孙慧涌朱林丁娟冯志彬刘林德
Owner LUDONG UNIVERSITY
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