Method for identifying genuine Chinese medicine based on stability isotope element fingerprint pattern
A technology of fingerprints and Chinese herbal medicines, which can be used in the separation of materials, analysis of materials, preparation of test samples, etc., and can solve the problem of inaccurate origin of Chinese herbal medicines.
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[0023] Embodiment 1: The construction of the stable nitrogen and hydrogen isotope fingerprints of part of Henan Daodi Chinese medicinal material Huai Yam is described below, and the method is described, and the concrete method steps are as follows:
[0024] 1. Procedure for the determination of stable nitrogen isotopes
[0025] 1. Sample collection
[0026] Due to the specificity of plant samples, every detail of sampling must ensure minimal error in the final assay results. When sampling plants, pay attention to the following issues:
[0027] (1) When sampling, in order to make the samples of traditional Chinese medicinal materials more representative, 5-10 individual plants of each species in each plot should be sampled separately, and then combined into one to form one of the species. sample.
[0028] (2) Since the isotopic fractionation of the N assimilation process in young plants and immature tissues has not been completed, 15 The natural abundance of N has not final...
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