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Improved methods of producing avian eggs and birds of specified germ-free status

A poultry and state technology, applied in poultry industry, reproductive cells, animal cells, etc.

Inactive Publication Date: 2009-05-06
OVEJDZHEN INTERNESHNL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, these methods still need to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] method

[0102] Flocks of fifty adult hens and five adult males in known specific pathogen free (SPF) status were maintained on a selected diet and allowed to breed naturally. The time each hen laid eggs (egg laying) was recorded separately over a two-week period. Calculate the average egg-laying time (time, L) of each hen in a day. The instant of L-3h is calculated and the period from L-3 to L is named the derivation interval. This interval is the time in which a sterile surgical laparotomy is performed to remove the most developed egg in each bird.

[0103] For this procedure, birds are euthanized by neck disjoint and immediately prepared. Dip the birds in the disinfectant solution for 5 minutes. Feathers were removed from the ventral thorax and abdomen and the skin was sterilized with a 50% iodine in alcohol solution heated to 37°C. Each bird was then placed under a specially adapted surgical isolator sterilized with a 5% peracetic acid solution and containing s...

Embodiment 2

[0110] Following the protocol of Example 1, a series of additional studies were carried out over a period of 2 years in order to improve and refine the method of Example 1.

[0111]A total of 106 birds, divided into six flocks of different ages and genotypes, were used in a series of small studies (using 9 to 20 birds each) to derive sterile eggs. During this time course, both hatchability and microbiological sterility status of chicks varied significantly, with hatchability ranging from 0 to 40%. This is significantly lower than previous results. Another bad factor was the failure of some studies to obtain sterility due to microbial contamination during egg harvesting. Therefore, the time it takes to obtain eggs after induction of anesthesia or euthanasia is usually increased in order to ensure sterility.

Embodiment 2A

[0115] The effect of the time lag between expected spawning and aseptic surgical removal of eggs from parent birds on egg viability and sterility and ease of manipulation.

[0116] result

[0117] Hatchability: control (naturally produced eggs), 85-100% hatched, 80-100% sterile;

[0118] Eggs removed from the uterus within 30 minutes of anesthesia or euthanasia, 13-40% hatched, 80-100% sterile;

[0119] Of the eggs removed from the uterus 60 minutes after euthanasia, 14% hatched and 80-92% were sterile.

[0120] These results established that the viability of pre-mature eggs in hens decreased over time after euthanasia.

[0121] Ease of operation:

[0122] Control, not applicable;

[0123] The above 30 minutes, good, the organization is easy to improve;

[0124] Above 60 minutes, difficult, difficult tissue lifting / early postmortem stiffness.

[0125] These results established the convenience of aseptic surgical egg removal in a sterile surgical isolator by completing th...

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PUM

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Abstract

The invention provides an improved method of rearing a bird of specified germ- free status. Specifically, the present invention is directed to a method for increasing the hatchability and viability of surgicaliy derived eggs of germ-free status when obtained from a parent bird. The method comprises housing a bird as a parent bird, determining the position of a premature egg in the reproductive tract and stage of development of the premature egg in terms of shell formation and calcification, surgically removing an egg in its shell from the parent bird prior to transfer of the egg to the cloaca in the parent bird, incubating the egg and hatching the egg to produce a laying bird. The invention also relates to the production of avian eggs of specified germ-free status and uses thereof.

Description

technical field [0001] The present invention relates to an improved method of breeding poultry without specific bacteria status. In particular, the present invention relates to a method of increasing the hatchability and survival of eggs that have been aseptically removed from the peritoneal cavity of a parent bird and are in a sterile state. The invention additionally relates to the production of poultry eggs in a specific bacterial state. [0002] In this specification, the terms "uncontaminated" and "sterile" are used interchangeably. These terms are used very broadly and refer to a number of pathogens and infections that can be carried by birds, especially poultry such as chickens, turkeys, and other birds that are widely used to produce breeding birds flocks to produce fertilized eggs for commercial production, and to produce eggs and meat for human consumption. Additionally, these eggs and birds are used to produce a wide variety of biological substances, including va...

Claims

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Application Information

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IPC IPC(8): A01K45/00C12N5/071C12N5/075
CPCA01K67/02A01K45/00A61K31/47C12N5/0609
Inventor L·莫兰
Owner OVEJDZHEN INTERNESHNL
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