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Process for producing poxviruses and poxvirus compositions

A composition, a poxvirus technology, applied in the preparation of poxviruses, the use in the preparation of medicines, and the field of extracellular enveloped viruses

Active Publication Date: 2012-09-05
TRANSGENE SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of this, the existing poxvirus purification methods only target the virus in the packaging cells (that is, IMV), and the EEV particles shed into the culture medium are discarded

Method used

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  • Process for producing poxviruses and poxvirus compositions
  • Process for producing poxviruses and poxvirus compositions
  • Process for producing poxviruses and poxvirus compositions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0109] 1. Preparation of a composition comprising IMV and EEV.

[0110] A. Preparation of CEFs.

[0111] 66 SPF eggs were incubated in 2% formaldehyde solution for 60 seconds. After rinsing with 70% ethanol, the eggs were opened, and the embryos were removed and dissected. The resulting tissue was then digested with dispase (Ul / ml) and triple select (Ul / ml) at 36.5°C for 120 minutes.

[0112] The mixture was filtered to remove undigested tissue and CEFs were collected by centrifugation (2300 rpm, 15 minutes).

[0113] B. CEF culture and infection.

[0114] CEFs were incubated at 36.5° C. for 2 days in 55 1 VP-SFM (invitrogen). The cell culture medium was discarded, and poxvirus (0.05 MOI) was added to 55 l of Eagle basal medium (Invitrogen). The infected packaging cells were then incubated for three days.

[0115] C. Poxvirus purification.

[0116] Collect packaging cells and cell culture fluid. Mixture then at Silverson Homogenize in a L4RL4R high-speed homogenizer ...

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Abstract

The present invention relates to compositions and pharmaceutical compositions comprising poxviruses and more particularly extracellular enveloped viruses. The present invention also relates to a process for producing poxviruses and poxviruses obtained thereof. Moreover, the present invention also relates to the use of said poxvirus and said composition for the preparation of a medicament.

Description

technical field [0001] The present invention relates to compositions and pharmaceutical compositions comprising poxviruses, more particularly, extracellular enveloped viruses. The invention also relates to methods for preparing poxviruses and poxviruses obtained therefrom. In addition, the present invention also relates to the use of the poxvirus and the composition in the preparation of medicines. Background technique [0002] The emergence of new threats (avian influenza, West Nile virus, anthrax, etc.) and the development of gene therapy have increased the need to prepare and purify poxviruses for prophylactic or therapeutic purposes. This is especially true for the mammalian virus Ankara (MVA). The poxvirus was originally used to vaccinate immunocompromised patients against smallpox and is now also used as a vector for gene therapy. For example, MVA, as a carrier of MUCl gene, is used to inoculate patients to prevent tumors expressing Mucl (Scholl et al., 2003, J Biom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/863C12N5/06A61K48/00A61K35/76A61K39/00C12N7/02
CPCC12N7/02C12N2710/24143A61K2039/5256C12N2501/11C07K14/82A61K39/12A61K48/00C12N7/00C12N2500/24C12N2510/02C12N15/86C07K2319/01C12N2710/24151A61K35/76A61K39/0011A61K2039/55533A61K2039/585C12N2710/20034A61P31/12A61P35/00C12N15/863
Inventor D·马拉梅Y·克尔迪尔C·希恩
Owner TRANSGENE SA