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Anti-soman antibody

An antibody and sequence technology, applied in the direction of antibodies, anti-toxins, and the use of vectors to introduce foreign genetic materials, etc., can solve the problems of application limitations, difficulty in preparing human antibodies, and inability to immunize

Inactive Publication Date: 2011-05-18
PLA NAVY GENERAL HOSIPTAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the heterogeneity of mouse monoclonal antibody, it cannot directly immunize the human body, so its application in the human body is limited, and the preparation of human antibody is very difficult

Method used

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1, the acquisition of the antibody of anti-Soman

[0025] 1. Preparation of working library (large-capacity phage antibody library)

[0026] For specific preparation methods, please refer to the following literature: Sblattero D, Bradbury A. Exploiting recombination in single bacteria to make large phage antibody libraries. Nat Biotechnol, 2000; 18: 74.

[0027] From the original phage antibody library (Qiao Yuanyuan, Wang Yan, Chen Xiaosui, Wang Yuxiao, Hua Bing. Construction and identification of large-capacity phage antibody library, Chinese Journal of Microbiology and Immunology, 2004, 24(3), 194-197. China People's Liberation Army Naval General Hospital) took out 7×10 12 Phage particles of cfu superinfect BS1365 cells with a multiplicity of infection (MOI=100 / 1) of 100:1 (Wang Yan, Wang Yuxiao, Chen Xiaosui, Hua Bing, Liu Xiaolin, expression vector for large-capacity phage antibody library Construction and identification, Chinese Journal of Immunology,...

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PUM

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Abstract

The invention discloses an antibody against soman. The antibody against soman comprises a variable region of light chain and a variable region of heavy chain; wherein, the amino acid sequence of the variable region of heavy chain is the amino acid residue on the 133rd-246th position from the amino terminal of sequence 1 in a sequence table; the amino acid sequence of the variable region of light chain is the amino acid residue on 1st-111th position from the amino terminal of the sequence 1 in the sequence table. The antibody takes the immobilized P6-BSA as antigen and successfully screens humanized antibody against soman out by utilizing the constructed high-capacity phage antibody library; according to the evaluation of ELISA, the antibody against soman has good specificity and the combination activity with soman is 0.78 to 0.80 when the absorbency value is 490nm.

Description

technical field [0001] The present invention relates to an antibody against Soman. Background technique [0002] Soman is an organic phosphate compound, which can cause a series of central and peripheral cholinergic symptoms by irreversibly inhibiting the activity of acetylcholinesterase. It has the characteristics of strong toxicity, fast action, difficult to prevent and treat. Therefore, the medical protection of soman poisoning has attracted much attention, and it is one of the focuses of chemical defense medicine research. For soman poisoning, binding free soman with a specific antibody can avoid or reduce the inhibitory effect of the poison on cholinesterase, and prevent or even treat soman poisoning. [0003] The traditional hybridoma technology to prepare monoclonal antibodies is to link haptens with carrier proteins, and directly immunize mice to produce murine antibodies. Because of the heterogeneity of mouse monoclonal antibody, it cannot directly immunize the hu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/44C12N15/11C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A61K39/395A61P39/02
Inventor 周丽君王欲晓王玉霞杨日芳王琰贾蓓媛
Owner PLA NAVY GENERAL HOSIPTAL