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Method for separating tobacco total alkaloid from extract liquid of tobacco leftovers

A technology of tobacco waste and total alkaloids, applied in the direction of alkaloids, alkaloids, chemical instruments and methods, etc., can solve the problems of reducing the water solubility of nicotine, high equipment requirements, low transfer rate, etc., and achieve the reduction of recycled resin column process, high alkaloid transfer rate and simple process

Inactive Publication Date: 2009-07-29
BEIJING HERUN INNOVATION PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method avoids the use of a large amount of organic solvents, and has a certain degree of progress, but there are still serious defects: in the test, it can be found that the method can elute the total alkaloids of tobacco by mixing lye, although the purity of alkaloid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 1000g of tobacco waste, add water and decoct twice, 10 times the amount each time, decoct for 2 hours, combine the decoction liquid, centrifuge, adjust pH=3, centrifuge, and the supernatant is passed through cation exchange at 4 times the column volume / hour Resin (001×7, hydrogen type, diameter-to-height ratio 1:8), washed with water until the effluent is colorless, then eluted with 15% hydrochloric acid solution, the elution rate is 2 times the column volume / hour, until the alkaloid test is negative (The effluent was added dropwise with 10% silicotungstic acid without precipitation), the eluate was collected, neutralized to neutral with sodium hydroxide, desalted, and the filtrate was concentrated and dried to obtain total tobacco alkaloids.

Embodiment 2

[0036] Get tobacco waste 1000g, add the acidic water of pH=5 to infiltrate, combine the percolation liquid and add water and dilute to 10000ml, centrifuge, adjust pH=1, centrifuge, supernatant passes through cation exchange resin (Doulex 50, sodium type, diameter-to-height ratio 1:5), wash with water until the effluent is colorless, then elute with 5% hydrochloric acid solution, the elution rate is 4 times the column volume / hour, after elution is 2 times the column volume, soak for 5 hours , and then eluted until the alkaloid test was negative (the effluent was added dropwise with 10% silicotungstic acid without precipitation), the eluate was collected, neutralized to neutral with calcium hydroxide, desalted, the filtrate was concentrated and dried to obtain the total alkaloids of tobacco .

Embodiment 3

[0038] Take 5kg of tobacco waste, add 70% ethanol and ultrasonically extract twice, add 5 times the amount each time, ultrasonically for 0.5 hours, combine the extracts, centrifuge, recover ethanol, add water to 5000ml, adjust pH = 2, centrifuge, and the supernatant with 2 times the column volume / hour, pass through the upper cation exchange resin (001×10, sodium type, diameter-to-height ratio 1:7), wash with water until the effluent is colorless, and then wash with 1% hydrochloric acid solution (containing 1% sodium chloride) Elution, the elution rate is 5 times the column volume / hour, until the alkaloid test is negative (10% silicotungstic acid is added dropwise to the effluent without precipitation), the eluate is collected, neutralized to neutral with sodium hydroxide, and desalted , and the filtrate was concentrated and dried to obtain total tobacco alkaloids.

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PUM

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Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from discarded tobacco leaves extract, which belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal that the discarded tobacco leaves extract is taken and filtered, the pH value of the discarded tobacco leaves extract is adjusted to be between 1 and 7; filtrate is added to a cation exchange resin column, eluted with water to remove impurities and then eluted by use of 0.5 to 15 percent acid liquid; and eluent is collected, added with alkali for neutralization and desalted so as to prepare tobacco total alkaloid. The method overcomes the deficiency that the prior art is low in extraction ratio, large in the amount of organic solvents, complex in process, incapable of mass production, and the like, and can efficiently separate high-purity tobacco total alkaloid from the discarded tobacco leaves extract.

Description

technical field [0001] The invention relates to a method for extracting and separating effective components of traditional Chinese medicines, in particular to a method for extracting and separating total tobacco alkaloids from tobacco leftover extracts, belonging to the field of traditional Chinese medicines. Background technique [0002] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature, are active ingredients of many Chinese herbal medicines, and are an important class of substances in nature. Tobacco originated in America, Oceania, and some islands in the South Pacific. At present, 66 species have been found, and only 2 species have been cultivated and utilized, namely common tobacco (N.tabacum.L.), also known as safflower tobacco, and yellow flower tobacco (Nrustica L.). As there are hundreds of millions of smokers today, tobacco has become a major economic crop worldwide. In the production of cigarettes, a large amount of t...

Claims

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Application Information

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IPC IPC(8): A61K36/81A61P3/04A61P9/00A61P25/00A61P25/28A61P25/34C07G5/00
Inventor 朴美善王广录
Owner BEIJING HERUN INNOVATION PHARMA TECH DEV
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