Domestic silkworm silk glandulae specificity expressive HLH transcription regulate and control factor BmHLH2

A technology of transcription regulation factor and silk, which is applied in application, genetic engineering, plant gene improvement, etc., can solve the problem of not finding the regulation factor, and achieve the effect of promoting the development of silk industry, increasing silk production, and improving silk quality

Inactive Publication Date: 2009-07-29
SOUTHWEST UNIVERSITY
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Problems solved by technology

The number of silk gland cells and the number of nucleus replication in silkworm are closely related to the silk production of silkworm. However, there are still relatively

Method used

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Embodiment

[0055] Through the information analysis of the silkworm gene chip, it was found that two genes corresponding to the probe number sw01142 were specifically expressed in the silk gland of the silkworm, and the coded protein number of the corresponding silkworm was Bmb017097, and the gene was named BmHLH2; its protein sequence was in the SMART domain The domain prediction of the prediction software showed that they were two proteins containing the HLH domain; then the BLAST program was run in NCBI to search for homologous genes, and the search results showed that the BmHLH2 protein had the highest similarity with the factors controlling the specialization of human muscle cells. Its value is E=3e-19; analysis results show that this transcription factor specifically expressed in silk gland is a new type of bHLH transcriptional regulator specifically expressed in silk gland of silkworm; according to the functional research of its homologous genes, it shows that they have an important ...

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Abstract

The invention relates to an HLH transcriptional regulator BmHLH2 applied to special expression of domestic silkworm silk gland. The HLH transcriptional regulator BmHLH2 is obtained by a series of biotechnology clones such as analyses of an expression profile of silk gland genes, manufacturing, hybridization and data analysis of genetic chips and the like. The HLH transcriptional regulator obtained in the invention is a novel transcriptional regulator applied to the special expression of the domestic silkworm silk gland, fluorescent quantitative PCR experiment indicates that the transcriptional regulator has important links with the development of the silk gland and differentiation of silk gland cells; an intervention study on the BmHLH2 gene indicates that the HLH transcriptional regulator plays a very important role in regulating and controlling cell differentiation, specialization of organs and the special expression of genes. The research findings are of great significance in improving silk qualify and quantity of domestic silkworms and promoting the development of silk industry.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a transcription regulation factor specifically expressed from the silk gland of silkworm silkworm. Background technique [0002] The silk gland is the only organ of the silkworm that can produce cocoon silk, and it is the biological basis of the entire silk industry. A silkworm usually grows and develops for about 25 days. In the larval stage, it eats about 22g of fresh mulberry leaves and synthesizes and secretes 0.5-1g of silk. Protein, especially in the middle and late stages of the 5th instar, silkworms can convert mulberry leaf protein into silk protein with amazing efficiency. Its protein synthesis ability and efficiency are the strongest and highest among all biological tissues and organs discovered so far. According to the preliminary speculation of protein synthesis, this high-efficiency protein synthesis not only requires the coordination of many genes, but also the expres...

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Application Information

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IPC IPC(8): C12N15/12C07K14/435A01K67/04
Inventor 刘春马三垣夏庆友林英徐汉福向仲怀
Owner SOUTHWEST UNIVERSITY
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