Multi-shell rhzomorph derivative, preparation and uses thereof

Abstract

The invention discloses a myriocin derivative and a preparation method thereof. The preparation method comprises the following steps: 9-fluorenylmethoxycarbonyl chloride is added to myriocin under the condition that pyridine and tetrahydrofuran solution provide a reaction environment, the 9-fluorenylmethoxycarbonyl chloride reacts with the myriocin to generate the myriocin derivative, the derivative introduces a group with ultraviolet characteristic absorption peaks to amino groups of the myriocin, and quantitative analysis can be carried out by high performance liquid chromatography, thus further quantitatively characterizing the myriocin. The method has reliable and accurate determination results, and can be used for determining myriocin content of traditional Chinese herbal medicines such as cicada fungus, Chinese caterpillar fungus and the like, determining preparations containing the cicada fungus or the Chinese caterpillar fungus and extracts thereof, and determining the myriocin content of biological samples.

Description

A kind of myriocin derivative and its preparation method and application technical field The present invention relates to a myriocin derivative, in particular to a myriocin derivative introduced into the amino group with a characteristic ultraviolet absorption peak group and a preparation method thereof, and also relates to the quantitative determination of the derivative of the myriocin Application of contocin in biological products. Background technique Myriocin has a variety of pharmacological effects. Studies by Fujita et al. have shown that myriocin has a significant two-way immunoregulatory effect, can block the pathway below the interleukin-2 receptor, inhibit the activity of serine palmitoyltransferase, and thus specifically inhibit the proliferation of T cells; Myriocins have a strong inhibitory activity on the induction of the same kind of cell barrier T cells, which is 10 to 100 times stronger than cyclosporin A in these aspects; in the mixed reaction of the sa...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that: myriocin is a compound without chromophoric groups, and the detection wavelength needs to be selected at the ultraviolet end, and the determination of the ultraviolet end is subject to much interference and the sensitivity is not high; the chemical composition of the sample is complex, multi-sphere The content of chitosin is low, and it needs to be separated and purified before it can be analyzed by HPLC, which is cumbersome to operate

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