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Detectable nucleic acid tag

A nucleic acid and nucleic acid sequence technology, applied to the labeling, measuring devices, and biological tests used in chemical analysis, can solve problems such as difficulty in applying high-throughput applications, tediousness, detection sensitivity, and specificity limitations

Active Publication Date: 2009-08-19
迪斯卡沃雷克斯公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each of these methods is cumbersome and difficult to adapt for high-throughput use
These traditional methods also have limitations in detection sensitivity and specificity

Method used

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Embodiment

[0084] Unless otherwise indicated, the practice of the systems and methods provided herein employs molecular biology, microbiology, genetic analysis, recombinant DNA, organic chemistry, biochemistry, PCR, oligonucleotide synthesis and modification, nucleic acid hybridization, and techniques in the art Traditional techniques in the relevant field mastered by the skilled person. These techniques are described in the references cited herein and explained in detail in the literature. See eg: Maniatis et al., (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press; Sambrook et al., (2001), Molecular Cloning: A Laboratory Manual. Third Edition, Cold Spring Harbor Laboratory Press; Ausubel et al., Current Protocols in Molecular Biology. John Wiley & Sons (1987 and updated annually); Gait (editor) (1984) Oligonucleotide Synthesis: A Practical Approach. IRL Press; Eckstein (ed.) (1991) Oligonucleotides and Analogues: A Practical Approach. IRL Press...

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Abstract

Provided herein are nucleic acid tags that are linked to, or capable of linking to, a protein of interest. In particular, the nucleic acid tags are oligonucleotides comprising a reporter function and a protein tagging function. Also provided herein, are nucleic acid tag compositions, kits and methods of use thereof.

Description

field of invention [0001] The subject matter provided by the present invention relates to nucleic acid tags linked to, or capable of linking to, a protein of interest. In particular, the subject matter provided by the present invention relates to oligonucleotides comprising a reporter function and a protein labeling function. The invention also provides nucleic acid label composition, kit and using method thereof. Background of the invention [0002] Traditional techniques to quantify and detect the presence of proteins include gel electrophoresis, Western blotting, ELISA-based immunosorbent assays, and protein microarrays. Each of these methods is cumbersome and difficult to adapt for high-throughput use. These traditional methods also have limitations in detection sensitivity and specificity. The present invention provides a nucleic acid tag, and a novel, highly sensitive and selective protein detection method using the nucleic acid tag. Brief description of the inven...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/62G01N33/68
CPCC12Q1/68G01N33/532G01N33/585C12Q2563/179C12Q2522/101C12Q2563/113C12Q2563/101C12Q1/6806G01N33/573G01N2458/00
Inventor 皮艾特罗·奇切里杰里米·亨特吉恩-迈克尔·A·莱利亚斯迈克·莫里森丹尼尔·特莱柏利萨·M·沃迪科卡
Owner 迪斯卡沃雷克斯公司