Flax monoploid cell suspension system culture medium

Abstract

The present invention discloses a flax monoploid cell suspension system culture medium, which can solve the problem of poor pollen callus dispersivity, small amount of single cells and large proportion of cell mass in the N6, B5 and MS suspension system culture media. Each liter of flax monoploid cell suspension system culture medium is synthesized from the following materials by weight: 2186.1mg of 1 / 2N6 macro-elements, 15.8mg of B5 microelements, 1-20mg of thiamine hydrochloride, 1-10mg of pyridoxine hydrochloride , 100-300mg of praline, 100-500mg of glutamine, 10-50mg of active carbon, 1.0mg of 2.4-D, 0.1mg of 6-BA, 20-40g of cane sugar and the balance of water; and the PH value of the synthesized culture medium is 5.5-6.0. The obtained monoploid embryogenic cell has canary yellow color, active cell division, good dispersivityand small proportion of cell mass; the embryogenic callus inductivity is improved greatly, and the regenerated globular callus has tight globular structure and finely granular structure on surface thereon.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a flax haploid cell suspension culture medium. Background technique [0002] With the continuous progress of flax anther culture research in my country, anther culture technology has been applied in the field of flax resistance breeding. Various types of mutants can be screened at the cellular level by utilizing the high-frequency variation produced during subculture of haploid cells. Since the mutant haploid has only one set of chromosomes, there is no chimera, and the dominant and recessive mutant shapes are expressed at the same time, and the mutant shape is stable, which can speed up the process of resistance breeding. At present, cell suspension culture has been widely used in the screening of resistant cells. Single-cell populations can be obtained through cell suspension culture, which is relatively uniform under the action of mutagens and selection agents, and is not easy to...

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Problems solved by technology

[0003] The present invention solves the problem that calluses are often used as screening materials in the screening of flax-resistant mutants. During the screening of mutants, the cells cannot be directly contacted with the culture medium, so that the cells cannot be evenly distributed. Under the effect of selection pressure, it is not conducive to the screening of mutants; N 6 , B 5 1. The dispersion of pollen callus in MS suspension medium is not good. Although the cell proliferation rate is fast, the number of single cells obtained is small and the proportion of cell clusters is large. A suspension of flax haploid cells is provided. The specific technical scheme to solve this problem is as follows:

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