A kind of extraction method of Radix Polygoni Multiflori polysaccharide

Abstract

The invention discloses a method for extracting polysaccharides from Radix Polygonum multiflorum, belonging to the technical field of plant purification. The method comprises the following steps: uniformly disperse the powder of Radix Polygoni Multiflori in the alkyl glucoside solution, conduct cyclic ultrasonic extraction at 40-70° C. for 20-50 minutes, conduct precipitation treatment after the extraction, and collect the supernatant; evaporate the supernatant Concentrate the volume of the supernatant to 1 / 4-1 / 3, remove the protein, and then uniformly disperse in the ethanol solution, carry out alcohol precipitation at 4°C, and collect the alcohol precipitation solution; then, the alcohol precipitation solution is subjected to precipitation treatment, And after the sediment is treated, the polysaccharide of Radix Polygoni Multiflori can be obtained. Based on the cyclic ultrasonic extraction technology, the present invention adds alkyl glycosides as the extraction solvent, which can increase the extraction rate of Polygonum multiflorum polysaccharide from 1.45% of the traditional process to 2.15%, which is nearly 1.5 times higher, and the cost is greatly reduced. The extraction time Compared with the original method, the time is shortened by two-thirds, and the extraction temperature is suitable to maintain the activity of the polysaccharide.

Description

technical field [0001] The invention belongs to the technical field of plant purification, and in particular relates to a method for extracting polysaccharides from Radix Polygoni Multiflori. Background technique [0002] Cynanchum auriculatum is a plant of the genus Cynanchum in the family Asclepiadaceae. It tastes sweet, bitter, and mild in nature. It has the functions of nourishing liver and kidney, nourishing essence and blood, anti-aging, and blackening hair. Because of its anti-oxidation, anti-aging, and free radical scavenging effects, Baishouwu has been regarded as a treasure for health care and anti-aging by famous experts of all dynasties. 95% of China's Baishouwu is produced in Binhai County, Yancheng City. Studies have shown that Binhai Baishouwu is rich in protein, starch, vitamins, amino acids, inorganic salts and other nutrients, as well as C 21 Active ingredients such as steroids, benzophenones, phospholipids and polysaccharides. However, the current resear...

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Problems solved by technology

However, the current research on it is mainly focused on C 21 In terms of steroid extraction and component analysis, there are relatively few studies on the extraction process and physical and chemical properties of polysaccharides from Polygonum multiflorum

[0003] With the popularization of Baishouwu, people's acceptance of it has greatly increased, and more processed products of Baishouwu have gradually appeared on the market, such as Baishouwu powder fermented milk, Baishouwu fine powder, etc., but deep-processed products are still Very rare, Baishouwu industry still has problems such as insufficient research and development capabilities, weak brand effect, unsalable products, and low product grades that need to be resolved urgently

According to the survey, people still have doubts about the toxicity of Baishouwu products, and there is no research to clarify whether the nutrients in Baishouwu products interfere with each other, so the true market value of Baishouwu cannot be reflected

[0004] At present, the common extraction methods of plant polysaccharides in the market include water extraction method, enzymatic hydrolysis-assisted method, microwave-assisted extraction method, etc., but these extraction methods are demanding, complex extraction methods, long extraction cycle, less extraction amount and easy to destroy the activity of polysaccharides during the extraction process.

Due to the disadvantages of high cost and low yield of traditional technology, the polysaccharide of Polygonum multiflorum is still blank in the market

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