De novo formation and regeneration of vascularized tissue from tissue progenitor cells and vascular progenitor cells

A technology of progenitor cells and vascularization, applied in tissue culture, filters in blood vessels, microorganisms, etc., can solve the problems of suboptimal anastomosis of cost and slow endothelial migration

Inactive Publication Date: 2009-09-16
UNIV COLUMBIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there have been concerns about the cost of growth factor delivery, potentia...

Method used

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  • De novo formation and regeneration of vascularized tissue from tissue progenitor cells and vascular progenitor cells
  • De novo formation and regeneration of vascularized tissue from tissue progenitor cells and vascular progenitor cells
  • De novo formation and regeneration of vascularized tissue from tissue progenitor cells and vascular progenitor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1: Spatially co-seeded endothelial cells with MSC osteoblasts generate vessel-like structures in engineered bone constructs in vivo

[0107] Human bone marrow samples (AllCells, Berkeley, CA) were prepared according to previously established methods (Shi et al., 1998; Alhadlaq et al., 2004; Yourek et al., 2004; Marion et al., 2005; Moioli et al., 2006; Troken and Mao, 2006) isolated mesenchymal stem cells (MSC) and hematopoietic stem cells (HSC). figure 1 A depicts the bone marrow content of the initial plating showing a dense population of cells known to be heterogeneous. (See Alhadlaq and Mao, 2004; Marion and Mao, 2006).

[0108] Mesenchymal stem cells differentiate into osteoblasts. Two distinct cell lineages, human mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs), were used for vascularized bone engineering in vivo.

[0109] MSCs were isolated from human bone marrow samples as described above (see e.g. figure 1 B) (Alhad...

Embodiment 2

[0115] Example 2: Differentiation of bone marrow-derived hematopoietic stem cells into endothelial cells in vitro.

[0116] For clinical applications, HSCs can be isolated from bone marrow along with MSCs, preferably by a minimally invasive route. HSCs have been found to undergo slow expansion (Shih et al., 2000; Li et al., 2004). FGF-2 has been shown to accelerate the rate of HSC expansion (Wilson and Trump, 2006; Yeoh et al., 2006). The inventors' experience is that HSCs do expand at a slower rate than MSCs and HUVECs. Alternatively, HSCs can be differentiated into endothelial cells followed by expansion of HSC-derived endothelial cells.

[0117] Bone marrow samples (supra) were prepared for HSC isolation. CD34 and magnetic bead separation were used to isolate non-adherent cells (EasySep. AllCells, Berkeley, CA). The isolated CD34 positive cells (CD34+) were considered as HSCs. In fibronectin-coated plates, HSCs appear as round cells (see e.g. image 3 A), whose shape ...

Embodiment 3

[0121] Example 3: Growth factors induce angiogenesis in polymer hydrogels in vivo.

[0122] It has been demonstrated herein that HSCs and MSCs are capable of differentiating into definitive cell lineages such as endothelial cells and osteoblasts, which constitute some of the building blocks of blood vessels and bone. It was also demonstrated that blood vessel-like structures can be built in vivo within bone scaffolds. However, existing literature has shown that engineered blood vessels can be leaky due to abnormally high endothelial cell permeability (Richardson et al., 2001; Valeski and Baldwin, 2003). To determine the effect of bFGF on host-derived angiogenesis, the angiogenic factor bFGF was delivered into a dense polymer hydrogel, poly(ethylene glycol) diacrylate (PEGDA), known in previous studies to be In vivo is impermeable to host-derived vascular cells (Alhadlaq and Mao, 2003; Alhadlaq et al., 2004; Alhadlaq and Mao, 2005; Stosich and Mao, 2006).

[0123] Suboptimal ...

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Abstract

It has been discovered that vascularized tissue or organs can be engineered by combined actions of tissue progenitor cells and vascular progenitor cells. Provided herein are compositions and methods directed to engineered vascularized tissue or organs formed by introducing tissue progenitor cells and vascular progenitor into or onto a biocompatible scaffold of matrix material. Also provided are methods of treating tissue defects via grafting of such compositions into subjects in need thereof.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Application No. 60 / 819833, filed July 10, 2006, and U.S. Provisional Application No. 60 / 824597, filed September 5, 2006, both of which are incorporated herein in their entirety for reference. [0003] Statement on Federal Funding for Research and Development [0004] A part of the present invention is made with the use of the National Institute of Biomedical Imaging and Bioengineering (National Institute of Biomedical Imaging and Bioengineering) and the National Institute of Dental and Craniofacial Research (National Institute of Dental and Craniofacial Research) research grant No. R01DE15291 and R01EB02332 carried out with government support. The government has certain rights in this invention. [0005] Incorporation by reference of the submitted material on the compact disc [0006] No. field of invention [0007] The present invention generally relates to the d...

Claims

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Application Information

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IPC IPC(8): A61F2/01C12N5/00C12P1/00C12N5/08
Inventor J·J·毛
Owner UNIV COLUMBIA
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