Methods for treating and reducing the incidence of newcastle disease
A Newcastle disease virus, Newcastle disease technology, applied in pharmaceutical formulations, antiviral agents, medical preparations containing active ingredients, etc., can solve the problems of undisclosed treatment, infection and killing of Newcastle disease
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Embodiment 1
[0079]
[0080] The formulation can be diluted in a ratio of 1-1300 with water or another suitable diluent to provide a more dilute composition for various applications such as spraying, nebulizing, as an aerosol or as a liquid formulation.
Embodiment 2
[0082] The safety and tolerability study of Composition 1 was carried out in chickens using different doses and routes of administration. The results show that Composition 1 is an effective and suitable liquid additive for poultry feed water, liquid additive for nasal drops or solid additive for poultry feed.
[0083] 132 approximately 7-day-old white Leghorn chickens were divided into 11 groups, given different forms and concentrations of Composition 1 corresponding to Tables 1(a)-1(c), and subsequently exposed to Newcastle disease Virus.
[0084]Tables 1(a)-1(c) summarize the dosage, route and concentration of the composition of the present invention administered to 132 white Legion chickens in the safety and tolerability study.
[0085] Table 1(a)
[0086] Group
chicken number
number of chickens
Route of administration
dose / concentration
1
12
1-12
feed (continuous)
high
2
12
13-24
feed (cont...
Embodiment 3
[0139] The undiluted antiviral composition of Example 1 was prepared, and the diluted composition was tested in VERO E6 cells and in 10-day-old embryonated chicken eggs for antiviral activity against NDV in 10-fold serial dilutions. In addition, placebo was similarly diluted and tested.
[0140] The continuous cell line VERO E6 (CRL-1586) was obtained from the American Type Culture Collection (Rockdale, MD) and was cultured in the presence of 2 mM L-glutamine, 1.5 g / L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 10% fetal bovine serum (InvitrogenCorp [Gibco], Carlsbad, CA) in Minimum Essential Medium (Eagle) at 37°C and 5% CO 2 conditions to reproduce. Cells were grown in T75 flasks (BD Biosciences, Franklin Lakes, NJ) and transferred to 96-well plates and grown to 90% confluency.
[0141] Will 1×10 3 Concentration of B1 / B1 strain of NDV (half tissue culture infectious dose (TCID 50 )) with seven 10-fold serial dilutions (from 1×10 -3 ...
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