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Chilling stress resistant gene PpNPL4 family proteinl of physcomitrella patens and protein and vector thereof

A low temperature resistance technology of Physcomitrella patens, which is applied in the fields of genetic engineering, plant gene improvement, and the use of vectors to introduce foreign genetic materials. It can solve the problems of affecting plant physiological metabolism, plant cell dehydration, and affecting plant cell osmotic pressure. To achieve the effect of improving stress resistance and high-efficiency stress resistance

Inactive Publication Date: 2009-11-18
CAPITAL NORMAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

The results of these stress factors all lead to dehydration of plant cells, affect the osmotic pressure of plant cells, and then affect the normal physiological metabolism of plants, and cause plant death in severe cases.

Method used

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  • Chilling stress resistant gene PpNPL4 family proteinl of physcomitrella patens and protein and vector thereof
  • Chilling stress resistant gene PpNPL4 family proteinl of physcomitrella patens and protein and vector thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Cultivation and cold stress treatment of Physcomitrella patens

[0017] Physcomitrella patens was cultured to about 15 leaves, moved to 0°C for 0, 3, 6, 12, 24, 48, and 72 hours, and samples were taken for cDNA-AFLP analysis.

[0018] Attachment: Formula and preparation of Physcomitrella patens culture medium:

[0019] Mass elements:

[0020] Ca(NO 3 ) 2 4H 2 O 0.8g / L

[0021] FeSO 4 ·7H 2 O 0.0125g / L

[0022] MgSO 4 ·7H 2 O 0.25g / L

[0023] Trace elements:

[0024] CuSO 4 ·5H 2 O 0.055mg / L

[0025] ZnSO 4 ·7H 2 O 0.055mg / L

[0026] h 3 BO 3 0.614mg / L

[0027] MnCl 2 4H 2 O 0.389mg / L

[0028] CoCl 2 ·6H 2 O 0.055mg / L

[0029] KI0.028mg / L

[0030] Na 2 MoO 4 2H 2 O 0.025mg / L

[0031] Glucose 5g / L

[0032] Ammonium tartrate 0.5g / L

[0033] Phosphate buffer 1ml / L

[0034] Trace elements were prepared into 1000X mother solution and stored at 4°C.

[0035] For solid basic medium, add agar powder at a concentration of 0.8%. ...

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Abstract

The invention relates to the field of biogenetic engineering, in particular to a chilling stress resistant gene PpNPL4 family proteinl of physcomitrella patens and coding protein thereof and a vector and a transgenic cell line containing the gene. A nucleotide sequence of the gene is shown as in SEQ ID No.1, and an amino acid sequence of the coding protein is shown as in SEQ ID No.3. The obtained gene has efficient stress resistance. By the conventional gene engineering technology, the gene can be used for culturing transgenic crops with high quality so as to improve the stress resistance of crops.

Description

technical field [0001] The invention belongs to the field of biogenetic engineering. Specifically, the invention relates to a low temperature stress resistance gene Pp NPL4 family protein 1 of Physcomitrella patens, a protein encoded by it, a carrier containing the gene, and a transgenic cell line. Background technique [0002] Low temperature, drought, and high salinity are important environmental stress factors that limit plant growth and development, geographical distribution, and morphogenesis. The results of these stress factors all lead to dehydration of plant cells, affect the osmotic pressure of plant cells, and then affect the normal physiological metabolism of plants, and cause plant death in severe cases. Through the study of some model plants, it is found that the functional proteins and transcription factors induced by these three stress factors interact with each other. Therefore, it is extremely necessary to seek green plants that can tolerate low temperature,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/63C12N5/10
Inventor 何奕昆王慧胡勇崔素霞孙铭明
Owner CAPITAL NORMAL UNIVERSITY
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