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Physcomitrella patens gene PpMTRS4 resistant to low-temperature stress, protein and vector thereof

A low-temperature resistant technology of Physcomitrella patens, applied in genetic engineering, plant genetic improvement, and the introduction of foreign genetic material using vectors, etc., can solve problems affecting plant physiological metabolism, plant cell dehydration, and plant death, and achieve high-efficiency resistance. Stress resistance, the effect of improving stress resistance

Inactive Publication Date: 2010-03-10
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The results of these stress factors all lead to dehydration of plant cells, affect the osmotic pressure of plant cells, and then affect the normal physiological metabolism of plants, and cause plant death in severe cases.

Method used

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  • Physcomitrella patens gene PpMTRS4 resistant to low-temperature stress, protein and vector thereof
  • Physcomitrella patens gene PpMTRS4 resistant to low-temperature stress, protein and vector thereof

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Embodiment Construction

[0013] 1. Cultivation and cold stress treatment of Physcomitrella patens

[0014] Physcomitrella patens was cultured to about 15 leaves, moved to 0°C for 0, 3, 6, 12, 24, 48, and 72 hours, and samples were taken for cDNA-AFLP analysis.

[0015] Attachment: Formula and preparation of Physcomitrella patens culture medium:

[0016] Mass elements:

[0017] Ca(NO 3 ) 2 4H 2 O 0.8g / L

[0018] FeSO 4 ·7H 2 O 0.0125g / L

[0019] MgSO 4 ·7H 2 O 0.25g / L

[0020] Trace elements:

[0021] CuSO 4 ·5H 2 O 0.055mg / L

[0022] ZnSO 4 ·7H 2 O 0.055mg / L

[0023] h 3 BO 3 0.614mg / L

[0024] MnCl 2 4H 2 O 0.389mg / L

[0025] CoCl 2 ·6H 2 O 0.055mg / L

[0026] KI0.028mg / L

[0027] Na 2 MoO 4 2H 2 O 0.025mg / L

[0028] Glucose 5g / L

[0029] Ammonium tartrate 0.5g / L

[0030] Phosphate buffer 1ml / L

[0031] Trace elements were prepared into 1000X mother solution and stored at 4°C.

[0032] For solid basic medium, add agar powder at a concentration of 0.8%. ...

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Abstract

The invention relates to the field of bio-genetic engineering, in particular to a physcomitrella patens gene PpMTRS4 resistant to low-temperature stress, a protein and a vector thereof. The cDNA genesequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence of the encoded protein of the gene is shown in SEQ ID No. 2. The gene obtained by the invention has efficient stress resistance. Through common genetic engineering techniques, high-quality transgenic crops can be cultivated by utilizing the gene so as to improve the stress resistance of crops.

Description

technical field [0001] The invention belongs to the field of biological genetic engineering, in particular, the invention relates to the low temperature stress resistance gene PpMTRS4 of Physcomitrella patens and its protein and carrier. Background technique [0002] Low temperature, drought, and high salinity are important environmental stress factors that limit plant growth and development, geographical distribution, and morphogenesis. The results of these stress factors all lead to dehydration of plant cells, affect the osmotic pressure of plant cells, and then affect the normal physiological metabolism of plants, and cause plant death in severe cases. Through the study of some model plants, it is found that the functional proteins and transcription factors induced by these three stress factors interact with each other. Therefore, it is extremely necessary to seek green plants that can tolerate low temperature, drought, and high salt stress to study the abiotic stress of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/63C12N5/10C07K14/415A01H1/00
Inventor 何奕昆王慧胡勇崔素霞孙铭明
Owner CAPITAL NORMAL UNIVERSITY
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