Method for single-time measuring ten amino bioactivators in organism

A technology of bioactive substances and organisms, applied in the field of determination of amino bioactive substances in organisms, can solve the problems of large sample size, increase of system error, increase of experiment cost, etc., save manpower and material resources, and reduce system error , cost reduction effect

Inactive Publication Date: 2009-11-18
SHANGHAI UNIV OF T C M
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Problems solved by technology

[0004] Epinephrine (E), norepinephrine (NE), dopamine (DA), serotonin (5-HT), histamine (HA), glutamic acid (Glu), asparagus in the neuroendocrine immune network Glycine (Asp), γ-aminobutyric acid (GABA), glycine (Gly) and triiodothyronine (T 3 ) and other ten kinds of amino biologically active substances are determined according to conventional detection methods: such as high performance liquid chromatography-electrochemical detection of epinephrine (E), norepinephrine (NE), dopamine (DA) and other catecholamine neurotransmitters; Use o-phthalaldehyde as a fluorescent derivative reagent for the

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  • Method for single-time measuring ten amino bioactivators in organism
  • Method for single-time measuring ten amino bioactivators in organism
  • Method for single-time measuring ten amino bioactivators in organism

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Embodiment Construction

[0020] The present invention will be further elaborated below in conjunction with the accompanying drawings and specific embodiments. These examples should be understood as only for illustrating the present invention but not for limiting the protection scope of the present invention. After reading the contents of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent changes and modifications also fall within the scope defined by the claims of the present invention.

[0021] A preferred embodiment of the present invention proposes a method for the one-time determination of ten amino bioactive substances in a living body, including the establishment of a high-performance liquid chromatography analysis method for amino bioactive substances in blood, and the determination of the effect on the body after taking a certain traditional Chinese medicine. The influence of these ten factors in the neuroend...

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Abstract

The invention belongs to the field of biochemistry detection, in particular to the measurement of bioactive molecules of neuroendocrine immunological network, particularly to a measurement method of amino bioactivators in organism (such as blood or brain tissue), in particular discloses a method for the single-time measuring of ten amino bioactivators in organism, which comprises: taking dansyl chloride as derivative reagent to perform single-time measuring on ten amino bioactivators such as epinephrine (E), noradrenalin (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), histamine (HA), gluetamic acid (Glu), aspartic acid (Asp), Gamma-aminobutyric acid (GABA), glycin (Gly) and trilute (T3) within 75min. The method is simple and rapid, can sensitively measure the concentrations of the ten amino bioactivators in organism fluid, and is suitable to the research requiring to completely analyze the ten amino bioactivators in the organism.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and in particular relates to the determination of neuroendocrine immune network biologically active molecules, especially the determination method of amino biologically active substances in organisms (such as blood or brain tissue). Background technique [0002] The overall regulatory effect of traditional Chinese medicine is closely related to the neuroendocrine immune network, while epinephrine (E), norepinephrine (NE), dopamine (DA), serotonin (5-HT), histamine (HA), gluten amino acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine (Gly) and triiodothyronine (T 3 ) and other ten amino bioactive substances are factors of great significance for the study of neuroendocrine immune network. [0003] Epinephrine (E), Norepinephrine (NE), Dopamine (DA), Serotonin (5-HT), Histamine (HA), Glutamic Acid (Glu), Aspartic Acid (Asp), Gamma -Aminobutyric acid (GABA), glycine (Gly) and ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N33/74G01N33/68G01N30/34
Inventor 俞仲毅季晓慧金勇施鑫伟
Owner SHANGHAI UNIV OF T C M
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