Method for single-time measuring ten amino bioactivators in organism

Abstract

The invention belongs to the field of biochemistry detection, in particular to the measurement of bioactive molecules of neuroendocrine immunological network, particularly to a measurement method of amino bioactivators in organism (such as blood or brain tissue), in particular discloses a method for the single-time measuring of ten amino bioactivators in organism, which comprises: taking dansyl chloride as derivative reagent to perform single-time measuring on ten amino bioactivators such as epinephrine (E), noradrenalin (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), histamine (HA), gluetamic acid (Glu), aspartic acid (Asp), Gamma-aminobutyric acid (GABA), glycin (Gly) and trilute (T3) within 75min. The method is simple and rapid, can sensitively measure the concentrations of the ten amino bioactivators in organism fluid, and is suitable to the research requiring to completely analyze the ten amino bioactivators in the organism.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and in particular relates to the determination of neuroendocrine immune network biologically active molecules, especially the determination method of amino biologically active substances in organisms (such as blood or brain tissue). Background technique [0002] The overall regulatory effect of traditional Chinese medicine is closely related to the neuroendocrine immune network, while epinephrine (E), norepinephrine (NE), dopamine (DA), serotonin (5-HT), histamine (HA), gluten amino acid (Glu), aspartic acid (Asp), γ-aminobutyric acid (GABA), glycine (Gly) and triiodothyronine (T 3 ) and other ten amino bioactive substances are factors of great significance for the study of neuroendocrine immune network. [0003] Epinephrine (E), Norepinephrine (NE), Dopamine (DA), Serotonin (5-HT), Histamine (HA), Glutamic Acid (Glu), Aspartic Acid (Asp), Gamma -Aminobutyric acid (GABA), glycine (Gly) and ...

AI Technical Summary

Problems solved by technology

[0004] Epinephrine (E), norepinephrine (NE), dopamine (DA), serotonin (5-HT), histamine (HA), glutamic acid (Glu), asparagus in the neuroendocrine immune network Glycine (Asp), γ-aminobutyric acid (GABA), glycine (Gly) and triiodothyronine (T 3 ) and other ten kinds of amino biologically active substances are determined according to conventional detection methods: such as high performance liquid chromatography-electrochemical detection of epinephrine (E), norepinephrine (NE), dopamine (DA) and other catecholamine neurotransmitters; Use o-phthalaldehyde as a fluorescent derivative reagent for the determination of amino acid neurotransmitters by high-performance liquid chromatography fluorescence detection; use radioimmunoassay for the determination of triiodothyronine, etc. On the one hand, due to the different methods, the sample size that needs to be tested It will be relatively large and increase the cost of the experiment; on the other hand, due to different detection methods, different sample pretreatment steps, and different detection principles, it will lead to an increase in the systematic error of the experiment when the changes in the detection indicators are analyzed uniformly.

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