Method of extracting total alkaloids in chinese caterpillar fungus and uses

A technology of total alkaloids and extraction methods, applied in the direction of medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc., can solve unrealistic problems, achieve small toxic and side effects, inhibit lipid peroxidation, and remove freedom base effect

Inactive Publication Date: 2009-11-25
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in daily life, it is unrealistic to prohibit drinking alcohol, so exploring and developing a method to reduce alcohol damage to the liver is one of the effective ways to solve the problem

Method used

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  • Method of extracting total alkaloids in chinese caterpillar fungus and uses
  • Method of extracting total alkaloids in chinese caterpillar fungus and uses
  • Method of extracting total alkaloids in chinese caterpillar fungus and uses

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Experimental program
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Effect test

Embodiment 1

[0020] (1) The fruiting bodies of Cordyceps militaris are dried until the water content is below 5%, pulverized, and passed through an 80-mesh sieve;

[0021] (2) Carry out 20kHZ ultrasonic-assisted extraction with the pulverized Cordyceps militaris according to the parameters in Table 1, and repeat the extraction 3 times; centrifuge to get the supernatant, and merge the supernatant three times; decompression distillation reclaims the solvent to obtain an extract;

[0022] (3) Dissolve the extract by the HCl solution added in Table 1, take the supernatant, and set the volume to 100ml; successively add 30ml ether and 30ml chloroform to the filtrate to fully shake and wash, and remove the organic phase (containing non- organic impurities of alkaloids);

[0023] (4) Adjust the aqueous phase solution obtained in step (3) to the pH shown in Table 1 with 10% NaOH, extract 3 times with 30ml chloroform, combine, concentrate and dry to obtain total alkaloids, and the extraction yield i...

Embodiment 2

[0038]Example 2 Protective effect of serum containing Cordyceps militaris alkaloids on ethanol-induced liver cell damage

[0039] Serum pharmacology of traditional Chinese medicine, as an experimental technique and research method, has unique advantages in the study of pharmacology of traditional Chinese medicine: it can eliminate the interference of the physical and chemical properties of the crude compound preparation of traditional Chinese medicine; avoid wrong conclusions that may be drawn from direct in vitro experiments; it can be true Reflecting the blood drug concentration and its changes in the body; the experimental results are in good agreement with the in vivo experiments.

[0040] Rats were given 1 mg·kg total alkaloids of Cordyceps militaris -1 , once a day, for 3 days in total, 2 hours after gavage, intraperitoneally inject 1% (W / V) pentobarbital sodium 20ml kg -1 , Abdominal midline incision, inferior vena cava blood collection, placed in sterile test tubes. ...

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Abstract

The invention discloses a method of extracting total alkaloids in chinese caterpillar fungus and uses. The extracting method includes digeratur of chinese caterpillar fungus ethanol solution, and using ultrasonic wave to extract total alkaloids in assistant, then proceeding vacuum concentration to the obtained extracting solution for producing extract, using HCl solution to dissolve the extract and adding ethyl ether, chloroform respectively to remove liposoluble substance, using NaOH to regulate solution pH, adding chloroform for extraction, condenseing the chloroform layer and drying to obtain the total alkaloids. The invention is provided with low process cost, high yield and convenient to operate. The invention provided total alkaloids of chinese caterpillar fungus has extensive novel uses in medicine for reversing hepatocyte damage caused by ethanol.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of active ingredients of traditional Chinese medicine and pharmacology of traditional Chinese medicine. It relates to a method for extracting total alkaloids of Cordyceps militaris and the application of the total alkaloids, technical background [0002] Cordyceps sinensis is a composite of the subsealet of the Cordyceps fungus and the dead body of the host bat moth larvae, commonly known as Cordyceps sinensis. According to traditional Chinese medicine books and documents, Cordyceps sinensis has the effects of strengthening the kidney, nourishing the lungs, benefiting essence and qi, and regulating all deficiency and damage. Due to the special and strict parasitic conditions and growth environment, the natural resources of Cordyceps sinensis are very limited. In addition, over-excavation and increasing demand in recent years have resulted in a shortage of Cordyceps resources and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/066A61P39/00A61P1/16
Inventor 徐大宝颜辉边旭云张九成陈星朱冬吉
Owner JIANGSU UNIV OF SCI & TECH
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