An apparatus and method for in vivo intracellular transfection of gene, sirna, shrna vectors, and other biomedical diagnostic and therapeutic drugs and molecules for the treatment of arthritis and oth

A plastic surgery, biomedical technology, applied in the direction of treatment, electrotherapy, etc., can solve problems such as inability to apply

Inactive Publication Date: 2009-12-02
RGT UNIV OF CALIFORNIA
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  • An apparatus and method for in vivo intracellular transfection of gene, sirna, shrna vectors, and other biomedical diagnostic and therapeutic drugs and molecules for the treatment of arthritis and oth
  • An apparatus and method for in vivo intracellular transfection of gene, sirna, shrna vectors, and other biomedical diagnostic and therapeutic drugs and molecules for the treatment of arthritis and oth
  • An apparatus and method for in vivo intracellular transfection of gene, sirna, shrna vectors, and other biomedical diagnostic and therapeutic drugs and molecules for the treatment of arthritis and oth

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[0042] Exemplary embodiments of the present invention include: 1) Low-intensity in vivo localized intracellular transfection of genes, siRNA, shRNA vectors and other biomedical diagnostic and therapeutic drugs and molecules for the treatment of arthritis and other orthopedic diseases Electric field network-mediated devices; 2) Combination therapy of two or more genes, siRNA, shRNA vectors, and other biomedical diagnostic and therapeutic drugs and molecules mediated by low-intensity electric field networks in arthritis and other orthopedic diseases and 3) a list of exemplary molecules that can be used in the method and employ the devices disclosed herein.

[0043] Safe and effective drug delivery is a critical factor in the disclosed therapeutic methods. It is known that localized drug delivery can not only significantly increase the drug concentration in targeted tissues and organs and enhance the efficacy, but also significantly reduce or avoid the systemic side effects of th...

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Abstract

An apparatus for in vivo intracellular transfection of gene, siRNA, shRNA vectors, and other biomedical diagnostic and therapeutic drugs and molecules for the treatment of arthritis and other orthopedic diseases in large animals and humans includes: a source of low voltage, short duration pulses in long duration bursts (LSEN); an electrode mesh system coupled to the source for generating distributed electric field network into a joint, including bones, cartilages, and related tissues; and means for transfecting the gene, siRNA, shRNA vectors, and other biomedical diagnostic and therapeutic drugs and molecules into a joint. The electrode mesh system includes alternatively arranged negative and positive electrodes in a first array which is capable of being inserted into a joint cavity, and either an alternatively arranged negative and positive or an all negative second electrode array which is positioned outside of the joint and in directly contact with overlying skin.

Description

[0001] related application [0002] This application is related to US Provisional Patent Application Serial No. 60 / 883,238 filed January 3, 2007, which is hereby incorporated by reference and claimed as the basis of priority under 35 USC 119. technical field [0003] The invention relates to a device and a device for in vivo transfection of genes mediated by high-efficiency low-intensity electric field networks, siRNA, shRNA vectors and other biomedical diagnostic and therapeutic drugs for the treatment of arthritis and other orthopedic diseases in large animals and humans method. Background technique [0004] In addition, more than 80% of drugs work inside the cell, or through the regulation of intracellular molecules. Effective gene therapy relies on the delivery of nucleic acids into cells to be effective. Both siRNA and shRNA need to be delivered into cells to be effective. The lack of efficient intracellular gene, siRNA and shRNA delivery methods is a major obstacle ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61N1/30
CPCA61N1/325A61N1/327
Inventor L·森
Owner RGT UNIV OF CALIFORNIA
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