High pass tissue grinder and grinding method

A grinding method and a grinding machine technology, which are applied in the grinding field, can solve the problems of poor grinding effect and complex structure of raw material samples, and achieve the effects of less cross infection, simple structure and improved grinding efficiency.

Inactive Publication Date: 2010-02-17
NINGBO SCIENTZ BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current grinding machines have complex structures, and the effect of grinding raw material samples is not good.

Method used

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  • High pass tissue grinder and grinding method
  • High pass tissue grinder and grinding method
  • High pass tissue grinder and grinding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: A high-throughput tissue grinder, which includes a motor 1, a bracket 3 for placing the sample box 2, and a swing mechanism. The motor 1 is connected to the bracket 3 through the swing mechanism, and the bracket 3 is provided with a device that can hold the sample box 2. A fixed fixing device, the sample box 2 is provided with a cover plate 5 that can fix the sample tube 4 . The sample box 2 comprises an upper fixed plate 6 and a lower fixed plate 7, a support column 8 is arranged between the upper fixed plate 6 and the lower fixed plate 7, a plurality of first tube holes 9 are arranged on the upper fixed plate 6, and a plurality of first pipe holes 9 are arranged on the lower fixed plate. 7 is provided with a second tube hole 10 corresponding to the first tube hole 9 . The swing mechanism includes an eccentric wheel 11 and a drive shaft 12 , the eccentric wheel 11 is arranged on the output shaft of the motor 1 , and the eccentric wheel 11 is connected wit...

Embodiment 2

[0023] Embodiment 2: a grinding method of a high-throughput tissue grinder, which is characterized in that it comprises the following steps: (1), pouring the raw material sample 15 into the sample tube 4; (2), putting the sample tube 4 into 3 ceramic grinding balls 16, the diameter of the ceramic grinding balls 16 is between 10 mm, the diameter of the sample tube 4 is greater than the diameter of the ceramic grinding balls 16 by 1 mm, and the sample tube 4 is sealed with a cover 17; (3), pour all the raw materials The sample tube 4 of the sample 15 is inserted into the first tube hole 9 and the second tube hole 10 of the sample box 2 respectively, and then the sample tube 4 is fixed in the sample box 2 with the cover plate 5; (4), the sample box 2 Immerse in liquid nitrogen and cool for 3 minutes; (5), put the sample box 2 with a plurality of sample tubes 4 into the support 3 of the grinder, and fix the sample box 2 in the support 3 with a fixing device; (6) , Start the grinde...

Embodiment 3

[0024] Embodiment three: a grinding method of a high-throughput tissue grinder, characterized in that it comprises the following steps: (1), pouring the raw material sample 15 into the sample tube 4; (2), putting the sample tube 4 into 5 ceramic grinding balls 16, the diameter of the ceramic grinding ball 16 is between 2-10mm, the diameter of the sample tube 4 is greater than the diameter of the ceramic grinding ball 16 2.5mm, then the sample tube 4 is sealed with a cover 17; (3), all The sample tube 4 poured into the raw material sample 15 is inserted into the first tube hole 9 and the second tube hole 10 of the sample box 2 respectively, and then the sample tube 4 is fixed in the sample box 2 with the cover plate 5; (4), the The sample box 2 is immersed in liquid nitrogen and cooled for 4 minutes; (5), the sample box 2 with a plurality of sample tubes 4 is placed in the support 3 of the grinder, and the sample box 2 is fixed in the support 3 with a fixing device; (6), start ...

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Abstract

The invention discloses a high pass tissue grinder and a grinding method. 1-5 grinding balls are placed into a sample pipe, and the diameters of the grinding balls are between 2 mm and 30 mm; a samplebox is immerged into liquid nitrogen to be cooled for 1 minute-2 minutes; the sample box reciprocates under the action of a swing mechanism; and the reciprocating times of the swing mechanism is 200times / minute-3000 times / minute. The invention has the advantages that the grinding balls are placed into the sample pipe, the sample pipe is vibrated in a reciprocating way along a vertical directionunder the action of the swing mechanism or along a horizontal direction or along an arc direction, the grinding balls impacts samples at high speed to complete the grinding of the samples, the frictional impact among the grinding balls can effectively lead to the smash of sample tissues or cells; the closed sample pipe is used so as to be difficult to cause cross infection among raw material samples; and in addition, multiple samples can be simultaneously fast and effectively ground within 2-4 minutes, thus the grinding efficiency is greatly enhanced.

Description

technical field [0001] The invention relates to a grinding method, in particular to a high-throughput tissue grinding machine and a grinding method. Background technique [0002] High Throughput Tissue Grinders can be used for dry, wet or cryogenic grinding and blending and homogenization of many different materials. Types of materials include plant tissue, animal tissue, cells, bacteria and yeast, among others. For the extraction of nucleic acids in large-throughput samples, it is first necessary to grind the samples sufficiently to break up the tissues or cells of the samples. The high-throughput grinding machine can not only help the majority of scientific research users to be freed from simple and repetitive physical labor, greatly reduce the workload, but also improve the grinding efficiency and quality. However, most of the current grinding machines have complex structures, and the effect of grinding raw material samples is not good. Contents of the invention [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28B02C17/14B02C17/24B02C17/18B02C17/20
Inventor 周芳
Owner NINGBO SCIENTZ BIOTECH
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