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Kit for screening IgA deficient patients

A kit and reagent technology, applied in the field of biochemistry, can solve the problems of radioactive pollution, unfavorable popularization and application, complicated operation, etc., and achieve the effects of large number of detection, reduction of blood transfusion risk, and improvement of safety

Inactive Publication Date: 2010-03-03
上海血液生物医药有限责任公司 +1
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is a method for IgA screening and detection using radioimmunity technology. This method has high requirements for the laboratory, has radioactive pollution, and is complicated to operate. It is not suitable for the screening work of blood donors and is not conducive to popularization and application.

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0019] The detection method is as follows:

[0020] Equipment: 96-well flat bottom plate, test tube, wet box, water bath box, microplate reader, absorbent paper, pipette

[0021] Dilute the coating antibody (50ul for one plate) 20 times with antibody diluent, then dilute to a final concentration of 200 times with double distilled water, and add 100ul of the diluted antibody to each well. Incubate overnight at 4°C, wash the plate 3 times with TW-PBS, and then add 200ul of blocking solution in a 37°C water bath for 30 minutes, then wash the plate 3 times, and store at 4°C.

[0022] Add 100ul of 10,000-fold diluted serum to be tested to each well of the flat bottom plate, and bathe in water at 37°C for 1 hour.

[0023] Dry the liquid in the wells, wash the plate 5 times with TW-PBS, and blot dry. Add 100ul of enzyme-labeled secondary antibody diluted 40,000 times to each well, and bathe in water at 37°C for 1 hour.

[0024] Dry the liquid in the wells, wash the plate 5 times wi...

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PUM

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Abstract

The invention relates to a kit for screening IgA deficient patients, which consists of a reagent and a box body, wherein the reagent consists of a sample diluent, an antibody diluent, a plate-washingliquid, a confining liquid, a stop solution, a coating antibody, an enzyme-labeled secondary body, a human IgA antigen standard substance and a TMB. The kit for detecting IgA can be applied to blood routine tests by clinical treatment institutions and blood stations, reduces blood transfusion risks for the IgA deficient patients, and improves the safety of blood transfusion.

Description

Technical field: [0001] The invention relates to the field of biochemistry, in particular to a detection technology for IgA deficiency, in particular to a screening kit for IgA deficiency. Background technique [0002] IgA is an immunoglobulin, which can be divided into IgA1 and IgA2 subclasses according to the difference of its α chain. The IgA2 subclass has two allotypes, A2m(1) and A2m(2), A2m(1) is more common in Caucasians, and A2m(2) is more common in blacks and Orientals. About 1 / 700 of Caucasians (approximately 1 / 18500 of Japanese) serum IgG and IgM levels are normal, while IgA is lower than 0.05g / L, which is called IgA deficiency. [0003] There are two main types of transfusion immune reactions caused by IgA. One is that patients with IgA deficiency can produce anti-IgA after receiving immune stimulation. If blood or blood products containing IgA are transfused, it will lead to transfusion reactions; Blood type incompatibility between subtypes and subtypes can al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 刘达庄冯明亮沈彤印彪
Owner 上海血液生物医药有限责任公司
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