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Rooting method of transgenic regenerated seedlings of super-sweet corn

A technology for super-sweet corn and regenerated seedlings, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of long rooting time, narrow adaptability, unsatisfactory rooting rate, etc., and achieve the effect of saving time

Inactive Publication Date: 2012-11-14
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problems of long rooting time, unsatisfactory rooting rate and narrow adaptability existing in the rooting method of the existing super sweet corn transgenic regenerated seedlings, the purpose of the present invention is to provide a super sweet corn with wide adaptability, fast rooting speed and good effect. High-efficiency rooting method for transgenic regenerated seedlings of sweet corn

Method used

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  • Rooting method of transgenic regenerated seedlings of super-sweet corn
  • Rooting method of transgenic regenerated seedlings of super-sweet corn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Carry out rooting culture with callus transgenic regenerated plantlet of sweet corn inbred line 1132

[0024] (1): Under sterile conditions, select regenerated plants with normal plant type and obvious main stem, and carefully remove the browned tissue at the base.

[0025] (2): Transfer the above-mentioned prepared plants into the root basal induction medium, the medium composition is: 1 / 2MS macroelement + 1 / 2MS trace element + 1 / 2 iron salt + inositol 125mg / L + VB 1 1.75mg / L+VB 6 0.5mg / L+ niacin 0.25mg / L+ glycine 2.0mg / L+NAA 0.05mg / L+ sucrose 10g / L+ plant gel 2.3g / L (or agar powder 7.0g / L)+PH5.8. When cultivating the 10th day, nearly 50% of the plants see root point growth ( figure 1 a).

[0026] (3): Transfer the plants with root points in (2) to the rooting medium containing NAA+IBA (0.05mg / L NAA+2.0mg / L IBA) (the rest of the ingredients are the same as above) for root elongation and growth , after 20 days, a large amount of roots were seen to gro...

Embodiment 2

[0028] Embodiment 2: Carry out rooting culture with callus transgenic regenerated plantlet of sweet corn inbred line 1132

[0029] (1): Under sterile conditions, select regenerated plants with normal plant type and obvious main stem, and carefully remove the browned tissue at the base.

[0030] (2): Transfer the above-mentioned prepared plants into the root basal induction medium, the medium composition is: 1 / 2MS macroelement + 1 / 2MS trace element + 1 / 2 iron salt + inositol 125mg / L + VB 1 1.75mg / L+VB 6 0.5mg / L + niacin 0.25mg / L + glycine 2.0mg / L + NAA 0.15mg / L + sucrose 10g / L + plant gel 2.3g / L (or agar powder 7.0g / L) + PH5.8. When cultivating the 4th day, nearly 48% of the plants saw root point growth ( figure 1 b).

[0031] (3): Transfer the plants with root points in (2) to the rooting medium containing NAA+IBA (0.15mg / L NAA+5.0mg / L IBA) (the rest of the ingredients are the same as above) for root elongation and growth , after 24 days, a large amount of roots were see...

Embodiment 3

[0033] Embodiment 3: Carry out rooting culture with callus transgenic regenerated plantlet of sweet corn inbred line 1132

[0034] (1): Under sterile conditions, select regenerated plants with normal plant type and obvious main stem, and carefully remove the browned tissue at the base.

[0035] (2): Transfer the above-mentioned prepared plants into the root basal induction medium, the medium composition is: 1 / 2MS macroelement + 1 / 2MS trace element + 1 / 2 iron salt + inositol 125mg / L + VB 1 1.75mg / L+VB 6 0.5mg / L + niacin 0.25mg / L + glycine 2.0mg / L + NAA 0.1mg / L + sucrose 10g / L + plant gel 2.3g / L (or agar powder 7.0g / L) + PH5.8. When cultivating the 6th day, nearly 55% of the plants saw root point growth (figure 1 c).

[0036] (3): Transfer the plants with root points in (2) to the rooting medium containing NAA+IBA (0.05mg / L NAA+2.0mg / L IBA) (the rest of the ingredients are the same as above) for root elongation and growth , after 21 days, a large number of roots were seen t...

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Abstract

The invention discloses a rooting method of transgenic regenerated seedlings of super-sweet corn, and the method has high rooting rate and wide applicability. The method adopts two steps for rooting, the formation of root bases is firstly induced in a root induction culture medium containing NAA, and then the growth culture of regenerated roots is continuously carried out by being transferred into a rooting culture medium containing NAA plus IBA. Compared with the existing method, the rooting method has short rooting time and fast speed, and the transplantation can be carried out after 25 days of rooting culture; the rooting rate is high, and a root system is developed; the transplantation survival rate is high; and the rooting method is applicable to a variety of gene types and is an important reform of the transgenic seedling rooting culture technology of the super-sweet corn. The method is also a valuable reference for the rooting of other plants.

Description

technical field [0001] The invention belongs to the technical field of corn tissue culture and transgene research, and in particular relates to a method for rooting transgenic regenerated seedlings of super-sweet corn. Background technique [0002] Tissue culture technology refers to the technology of cultivating protoplasts, suspension cells, plant tissues, etc. under sterile conditions using artificial medium. Tissue culture technology is widely used in the field of agriculture, and it is an indispensable part of transgenic technology, providing ideal receptor materials for genetic engineering; providing a new means for conventional plant improvement procedures, creating more quickly and efficiently New varieties of various crops and garden plants. [0003] In super sweet corn tissue culture and transgenic technology, the rooting technology of transgenic regenerated seedlings seriously restricts the development of super sweet corn genetic engineering. In the rooting stag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 祁喜涛胡建广孙思思
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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