Liquid double reagent diagnostic reagent kit for determining content of potassium ions in serum and blood plasma

Abstract

The invention relates to a liquid double reagent diagnostic reagent kit for determining the content of potassium ions in serum and blood plasma. By using the reaction principle that phosphoenolpyruvic acid (PEP) can be catalyzed by K+dependency pyruvate kinase (PK) to be converted into pyruvic acid and taking lactate dehydrogenase (LDH) as indicator enzyme by means of NADH, the reagent kit detects the reduction amount of the NADH at 340 nm and obtains the result that the change of the absorbance every minute (delta / min) is directly proportional to the content of K+, thereby establishing an enzyme coupling method for continuously determining the potassium ions. In addition, enzyme and a primer system required by slowly generating reduction type nicotinoyl coenzyme are added in a system andan enzyme-primer-coenzyme slow reaction system is formed in a reagent, so that the coenzyme can be slowly and circularly supplemented and the reagent can be stabilized when the concentration reaches a certain balance. The liquid double reagent diagnostic reagent kit is convenient and concise to use, carries out rapid detection on a common ultraviolet / visible light analyzer or a semiautomatic / fully-automatic chemical analyzer, needs no special or extra instrument and has low cost.

Description

technical field [0001] The invention relates to a liquid double-reagent diagnostic kit for measuring potassium ion content in serum and plasma by enzymatic method. Background technique [0002] Potassium in the human body is the main cation to maintain the physiological activities of cells, it is necessary to maintain the normal osmotic pressure and acid-base balance of the body, participate in the metabolism of sugar and protein, and ensure the normal function of neuromuscular. Potassium ions mostly exist in the cell, and a small amount exists in the extracellular fluid, and the concentration is relatively constant. Potassium in the human body mainly comes from food. The determination of serum potassium salt is actually the determination of potassium ions in extracellular fluid, but potassium ions in the body are constantly exchanged between cells and body fluids to maintain a dynamic balance. Therefore, the level of serum potassium concentration can also indirectly refle...

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Problems solved by technology

[0009] (6) Others: Aldosterone deficiency or long-term use of anti-aldosterone diuretics and familial hyperkalemic periodic paralysis can increase serum potassium

[0012] (1) Potassium intake is insufficient: long-term low potassium diet, fasting or anorexia, etc.

But there are disadvantages: it needs gas source and fire source, and the safety is poor; it needs repeated calibration and stability when starting up, and it is not convenient for individual detection of emergency specimens; it needs specific deionized water or lithium solution in the measurement, which is troublesome to test and difficult to store reagents; Unstable compressed air can directly affect the stability of the flame photometer; the protein and lipid in the sample can change the viscosity of the sample solution, affect the spray speed of the sample, and change the emitted light intensity of the flame, resulting in errors in the measurement results

[0027] Because the existing enzymatic reagents are all dry powder reagents, it is inconvenient to use and has poor stability after rethawing

It has a certain impact on clinical use. At the same time, the stable period after refusion is usually one week or less, which brings certain errors in the accuracy of sample detection. Therefore, the industry needs an easy-to-use, simple and easy-to-use UV Rapid detection on / visible light analyzer or semi-automatic / automatic biochemical analyzer, no need for special or additional equipment, and low-cost enzymatic potassium ion detection kit

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