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Method for culturing coriolus versicolor and inducing oxalate decarboxylase by rice straw carbon source

A technology of oxalate decarboxylase and Coriolus versicolor, which is applied in the field of preparation of oxalate decarboxylase, can solve problems such as air pollution and increased greenhouse effect, and achieve the effects of easy operation, simple method and high conversion rate

Inactive Publication Date: 2011-11-09
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the burned straw can be spread on the fields as fertilizer, the gas produced during the burning process will increase the greenhouse effect, and the smoke generated will also cause air pollution

Method used

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  • Method for culturing coriolus versicolor and inducing oxalate decarboxylase by rice straw carbon source
  • Method for culturing coriolus versicolor and inducing oxalate decarboxylase by rice straw carbon source
  • Method for culturing coriolus versicolor and inducing oxalate decarboxylase by rice straw carbon source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Production of Coriolus versicolor from rice straw hydrolyzate (the initial pH value of the medium is 5.0)

[0028] Weigh the fully ground rice straw, add a sulfuric acid solution with a concentration of 2% at a solid-to-liquid ratio of 1g: 12ml, soak the rice straw powder in a dilute sulfuric acid solution for 10 hours, place it in a high-pressure cooker, and heat it at 121°C , reacted for 30 minutes, filtered to remove the rice straw residue, and obtained the rice straw hydrolyzate for later use. The content of reducing sugar in rice straw hydrolyzate was determined by DNS method (glucose was used as standard sugar), and it was stored at 4°C for future use. Add rice straw hydrolyzate containing 1g reducing sugar (calculated as glucose) to every 50mL medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01gNa 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnSO 4 ·H 2...

Embodiment 2

[0032] Production of Coriolus versicolor from rice straw hydrolyzate (the initial pH value of the medium is 5.0)

[0033] Weigh the fully ground rice straw, add a sulfuric acid solution with a concentration of 3% at a solid-to-liquid ratio of 1g:8ml, soak the rice straw powder in a dilute sulfuric acid solution for 10 hours, place it in a high-pressure cooking pot, and heat it at 150°C , reacted for 60 minutes, filtered to remove the rice straw residue, and obtained the rice straw hydrolyzate for later use. The content of reducing sugar in rice straw hydrolyzate was determined by DNS method (glucose was used as standard sugar), and it was stored at 4°C for future use. Add rice straw hydrolyzate containing 0.35g reducing sugar (calculated as glucose) to every 50mL medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01gNa 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnSO 4...

Embodiment 3

[0036] Production of Coriolus versicolor from rice straw hydrolyzate (the initial pH value of the medium is 5.0)

[0037] Weigh the fully ground rice straw, add a sulfuric acid solution with a concentration of 3% at a solid-to-liquid ratio of 1g: 15ml, soak the rice straw powder in a dilute sulfuric acid solution for 10 hours, place it in a high-pressure cooker, and heat it at 100°C , reacted for 60 minutes, filtered to remove the rice straw residue, and obtained the rice straw hydrolyzate for later use. The content of reducing sugar in rice straw hydrolyzate was determined by DNS method (glucose was used as standard sugar), and it was stored at 4°C for future use. Add rice straw hydrolyzate containing 1.5g reducing sugar (calculated as glucose) to every 50mL medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01gNa 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnSO 4 ·H ...

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Abstract

The invention relates to a method for culturing coriolus versicolor and inducing oxalate decarboxylase by a rice straw carbon source. The method comprises the following steps of: (1) dipping pulverized rice straws by using an acid solution and hydrolyzing under the condition of 100-150 DEG C; (2) preparing a fermentation culture medium and regulating the pH value to be 4.0-5.0; (3) inoculating mycelia into the liquid seed culture medium, transferring both the mycelia and the culture medium into a container containing sterile glass beads when a white mycelium group is formed but does not emerge from the water level, shaking, smashing and inoculating the mycelia into the fermentation culture medium according to an inoculating quantity of 3-15 percent; (4) culturing for 4-6 days, adding oxalic acid and then culturing 0.5-6 days to obtain the mycelia; and freezing the mycelia by liquid nitrogen, then grinding, smashing, extracting mycelium fragments by an acetic acid buffer solution and centrifuging. The method is simple and easy to operate, and has low cost, strong controllability of carbon source preparation of and stable sugar content.

Description

technical field [0001] The invention belongs to the field of preparation of oxalate decarboxylase, in particular to a method for cultivating Coriolus versicolor as a carbon source of rice straw to induce oxalate decarboxylase. Background technique [0002] Oxalate decarboxylase can catalyze the decarboxylation of oxalate to form formic acid and carbon dioxide, so it has broad application prospects in the enzymatic detection of oxalate content, the degradation of oxalate in industrial wastewater, the preparation of low-oxalate-content foods, and the reduction of oxalate concentration in the human body. Coriolus versicolor is an important strain for producing oxalate decarboxylase, how to reduce its culture cost, produce mycelium in large quantities, and induce mycelium to synthesize oxalate decarboxylase is an important issue. Since carbon is the most abundant element in organisms, the carbon source is the most used in the medium and accounts for a relatively high proportion ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/88
Inventor 洪枫杨雪霞杨光曹张军
Owner DONGHUA UNIV
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