Method for screening low enrichment cadmium peanut varieties by fluorescent quantitative PCR technique

Abstract

The invention provides a method for screening low enrichment cadmium peanut varieties by a fluorescent quantitative PCR technique, which relates to a method for screening the low enrichment cadmium peanut varieties. The method comprises the following steps of: 1, culturing peanut seedlings by using nutrient solution; 2, designing specific different cadmium treatment levels; 3, performing a methodfor detecting and analyzing expression differences of AhMT2a genes of peanuts; and 4, screening cadmium sensitive varieties by using the method for detecting the expression differences of the genes by using the fluorescent quantitative PCR technique according to the screening bases of culturing the peanut seedlings by using the nutrient solution, and using sequences of the AhMT2a genes of leaf blades of the seedlings. The method has the advantages of simple detection method, no need of probe design, low cost and good universality, and can perform melting curve analysis to detect the specificity of amplified reactions.

Description

Technical field: [0001] The invention relates to a method for screening peanut varieties with low cadmium enrichment, in particular to a method for screening peanut varieties with low cadmium enrichment by fluorescence quantitative PCR technology. Background technique: [0002] Peanuts are one of the four major oil crops in the world, and are also the oil crops and economic crops with the highest yield and total yield in my country. The planting area exceeds 5 million hectares, and its output accounts for 1 / 2 of the total output of oil crops and 45 of the total output of peanuts in the world. %, has reached more than 14.5 million tons. For many years, peanuts have been the oil crop with the highest foreign exchange earnings in my country. At present, the main challenges facing peanut production in my country are aflatoxin pollution, pesticide residues (mainly butyl hydrazide pollution) and high levels of cadmium (Cd) in grains. Cd has attracted much attention due to its high tox...

AI Technical Summary

Problems solved by technology

However, the conventional screening methods include harvesting full peanuts through potted cadmium treatment, conducting atomic absorption measurement of cadmium content in seeds and measuring the differences in physiological responses of different peanut varieties to cadmium stress. However, there are many varieties, long growth cycles, and cumbersome operations. High cost, troublesome analysis and processing

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