Spectrophotometric determination method of sperm density of Russian sturgeon
A measurement method and spectrophotometric technology, applied in the direction of color/spectral characteristic measurement, measurement device, particle suspension analysis, etc., can solve the problems of inefficiency, cumbersome blood cell counting method, etc., and achieve good detection effect
Inactive Publication Date: 2011-09-21
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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[0004] Russian sturgeon is an important aquaculture fish in my country. When determining important parameters such as the optimal sperm-to-egg ratio in artificial insemination and the dilution ratio of semen in cryopreservation, it is necessary to accurately measure the sperm density. It is cumbersome and inefficient, and there is no relevant report on the determination of the sperm density of Russian sturgeon by spectrophotometry at home and abroad.
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[0009] The analysis equipment that the present invention adopts is Unocal UV-2802s ultraviolet-visible spectrophotometer or ordinary visible spectrophotometer.
[0010] The present invention finds that 530nm is the optimum detection wavelength through regression analysis on the sperm density and absorbance values under different detection wavelengths. Regression analysis was performed on the sperm density and absorbance values at different dilution ratios, and it was found that the effective detection density range was 3×10 6 ~1.5×10 9 / ml, beyond the detection range, the regression coefficient is low, and the error rate will increase.
[0011] Therefore, after diluting Russian sturgeon sperm 10 times with 0.9% NaCl solution, the absorbance value A was measured at a wavelength of 530nm 530 , sperm density C according to the following formula:
[0012] C = 10 A 530 ...
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Abstract
The invention discloses a spectrophotometric determination method of sperm density of Russian sturgeon, relates to a determination method of the sperm density of aquatic animals and aims at solving the technical problem of providing the spectrophotometric determination method of the sperm density of the Russian sturgeon. The technical scheme adopts a Unocal UV-2802s ultraviolet-visible spectrophotometer or an ordinary visible spectrophotometer as an analytic device, and the method is characterized in that the detection wavelength is 530nm, the density range for effective detection is 3*10<6>-1.6*10<9> / ml, 0.9% NaCl solution is used for diluting the sperm of the Russian sturgeon by 10 times, then the absorbance value A530 is determined at the wavelength of 530nm, and the sperm density C is calculated according to the formula C=10<((A530+8.560) / 1.323)>*10. The spectrophotometric determination method is used for determining the sperm density of the Russian sturgeon.
Description
technical field [0001] The invention belongs to a method for measuring sperm density of aquatic animals. Background technique [0002] Sperm density is an important indicator to reflect the quality of sperm. At present, the commonly used methods for measuring sperm density include hemocytometer method, Coulter particle counting method, flow cytometry method and spectrophotometry method, and a special method has been developed. Sperm Density Meter. Although the results of the hemocytometer method are more accurate, the workload is heavy when measuring a large number of samples; the instruments and equipment required by the Coulter particle counting method and the flow cytometry method are expensive; and the spectrophotometric method has the advantages of simple operation and low instrument price. And other advantages, using the established standard curve of sperm density and absorbance can quickly and accurately measure the sperm density, which has been applied in medicine a...
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IPC IPC(8): G01N15/06G01N21/31
Inventor 张涛庄平章龙珍刘鉴毅赵峰冯广朋黄晓荣侯俊利闫文罡田美平
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI