Molecular marker technology for identifying red grains and white grains of wheat

Abstract

The invention discloses a molecular marker technology for identifying red grains and white grains of wheat, which comprises the following steps: utilizing synthetic hexapliod wheat derived varieties of red grain wheat Chuanmai 42 and Sichuan cultivated white grain wheat Chuan W565 for hybridization, constructing an F2 population for genetic analysis of red grain genes, and harvesting 1015 F2 plants for identifying grain color and carrying out the genetic analysis and the molecular marking of the red grain genes of Chuanmai 42, wherein 241 white grain single plants in the F2 population are selected, and an F2-3 family is constructed for verifying the F2 result. The molecular marker technology utilizes an SSR modular marker for carrying out mapping on the red grain genes carried in the synthetic hexapliod wheat derived variety-Chuanmai 42, thereby being fast, accurate and effective to select the white grain variety, eliminating the red grain variety and accelerating the breeding process of the white grain wheat.

Description

technical field [0001] The invention relates to a technique for distinguishing wheat red grains and white grains by using SSR molecular markers, and belongs to the field of agricultural biotechnology engineering. Background technique [0002] Wheat kernel color is an important trait affecting flour quality and yield, and red wheat affects flour brightness during milling (Flintham, 2000; Warner et al., 2000; Himi et al., 2002), various countries in the world of farmers prefer to grow white wheat varieties. The color of wheat grains is controlled by red genes (R-A1, R-B1, R-D1) on the long arms of wheat chromosomes 3A, 3B, and 3D respectively. Red genes are dominantly inherited and have additive effects. Only when the sexual locus is pure can the white kernels be displayed, and the performance of the kernel color is affected by the environment, which makes it difficult to select white kernels with the naked eye, and the selection of white kernels can only be carried out after...

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Problems solved by technology

[0004] Aiming at the problem that white-grained wheat varieties are difficult to select with the naked eye, and can only be carried out after the seeds are harvested, and the cycle is long, the purpose of the present invention is to provide a molecular marker technology for distinguishing wheat red grains and white grains, using SSR molecular markers, Mapping the red-grain gene carried by the synthetic wheat derivative variety Chuanmai 42, in order to provide rapid and effective molecular markers for the breeding of white-grained wheat varieties using synthetic wheat and Chuanmai 42 as genetic resources

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