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Molecular marker AhyBA1 closely linked with peanut branching angle and application of molecular marker AhyBA1

A molecular marker, branching angle technology, applied in the field of agricultural biology, can solve the problems of increased molecular detection cost, high price of endonuclease, cumbersome process, etc., and achieves the goal of helping fine positioning, simple technical requirements, and high reliability. Effect

Inactive Publication Date: 2021-08-10
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CAPS tagging is an amplified polymorphic sequence tagging technology based on enzyme digestion. In the actual operation process, it is necessary to amplify by PCR first, then purify the amplified product, then digest the amplified product, and then perform electrophoresis. The process is relatively cumbersome, and the price of endonuclease is high, which will increase the cost of molecular detection

Method used

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  • Molecular marker AhyBA1 closely linked with peanut branching angle and application of molecular marker AhyBA1
  • Molecular marker AhyBA1 closely linked with peanut branching angle and application of molecular marker AhyBA1
  • Molecular marker AhyBA1 closely linked with peanut branching angle and application of molecular marker AhyBA1

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]Example 1: Location of key genes controlling peanut branch angle and design of molecular marker AhyBA1 closely linked to peanut branch angle

[0037] In order to locate the key gene controlling the branching angle of peanut, the inventors used the creeping peanut variety Tifrunner as the female parent to cross with the erect peanut variety Fuhuasheng (male parent). Phenotypic analysis showed that the branches of the creeping peanut variety "Tifrunner" grew close to the ground, and the angle between it and the main stem was 80° to 90°; The ground is completely separated from the main stem at an angle of 30° to 40°. its offspring F 1 The angle between the main stem and the parent stem is between 50° and 70°. A total of 70 hybrids of Tifrunner×Peanut F were harvested 1 seeds, using molecular markers for F 1 The plants were tested and found 25 true hybrid F 1 generation. f 2 A total of 332 F 2 single plant. According to F 2 According to the statistical results of t...

Embodiment 2

[0038] Example 2: Using the molecular marker AhyBA1 to rapidly cultivate a new high-yielding creeping peanut variety

[0039] By using the molecular marker AhyBA1 for selection combined with backcross breeding, the erect peanut (recurrent parent) can be improved into a creeping peanut in about 3 years, and more than 97% of the genetic background of the recurrent parent can be retained. On the premise of most of the excellent traits of the original peanut varieties, the directional improvement of the plant type is realized.

[0040] With the erect peanut Haihua No. 9 as the recurrent parent and the creeping peanut Tifrunner as the donor male parent, the specific steps for rapid improvement of peanut plant type are as follows:

[0041] (1) hybridization

[0042] The erect peanut variety Haihua No. 9 was used as the female parent (reincarnation parent) and the creeping peanut variety Tifrunner was used as the male parent for crossing. The hybridization method is as follows: ema...

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Abstract

The invention discloses a molecular marker AhyBA1 closely linked with a peanut branching angle and application of the molecular marker AhyBA1, and belongs to the technical field of agricultural biology. The specific primer pair of the molecular marker AhyBA1 comprises primers with nucleotide sequences shown as SEQ ID No.1 and SEQ ID No.2; the molecular marker disclosed by the invention can be used for rapidly, effectively and economically identifying the branching angle of the peanut, can realize directional rapid improvement of the plant type of the peanut through a method of combining auxiliary selection of the molecular marker with backcross breeding, and has an important application value in cultivation of creeping high-yield peanuts.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a molecular marker AhyBA1 closely linked to the peanut branch angle and its application. Background technique [0002] Peanut is an important oil and economic crop in my country, with an annual planting area of ​​about 70 million mu and an output of about 17 million tons. Improving peanut yield and realizing mechanized production are the main goals of peanut breeding and cultivation. Peanut is a crop that "flowers on the ground and bears fruit underground". The branching angle directly affects the needle penetration, swelling and pod yield, and is also closely related to planting density, cultivation methods and mechanized harvesting. Therefore, the branching angle is of great significance for improving peanut yield and realizing mechanization. [0003] According to the angle between the branch and the main stem and the ratio of the length of the branch to the main ste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 王兴军赵传志周希萌赵术珍李膨呈田锐铮侯蕾厉广辉夏晗潘教文
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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