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Preparation method of flavone genin of seabuckthorn leaves

A seabuckthorn leaf flavonoid aglycone and production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost and unfavorable industrial production, and achieve convenient storage, long picking cycle and high yield big effect

Active Publication Date: 2010-07-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, seabuckthorn flavonoids are converted into conformation through the action of enzymes, but the catalyst in the enzymatic conversion is an expensive glycoside hydro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of seabuckthorn leaf extract powder containing flavonoids: extract seabuckthorn leaves by shaking 80% methanol aqueous solution for 8 hours at 45° C., filter with suction, repeat three times, and combine the extracts. Then, the extract was freeze-dried to obtain seabuckthorn leaf extract powder, the content of flavonoids in the powder was 12%, and the content of total seabuckthorn flavonoids (that is, the sum of all existing forms of the three main aglycones) was 3.3%;

[0020] (2) Preparation of liquid medium: add ethylene glycol 15mL, orange peel powder 3g to 100mL water, (NH 4 ) 2 SO 4 2g, bran 2g, urea 0.075g, CaCl 2 0.015g, adjust the pH value to 5.0;

[0021] (3) Take 50 mL of the liquid medium in step (2) and inoculate it with Aspergillus niger (Asp. 5 a spore;

[0022] (4) Get 150 mg of seabuckthorn leaf extract powder that step (1) obtains and join in the liquid culture medium that is inoculated with Aspergillus niger in step (3), then pl...

Embodiment 2

[0026] (1) Preparation of seabuckthorn leaf extract powder containing flavonoids: extract seabuckthorn leaves by shaking 65% ethanol aqueous solution for 8 hours at 45° C., filter with suction, repeat three times, and combine extracts. Then, the extract was freeze-dried to obtain seabuckthorn leaf extract powder, the content of flavonoids in the powder was 15%, and the content of total seabuckthorn flavonoids (that is, the sum of all existing forms of the three main aglycones) was 4.7%;

[0027] (2) Preparation of liquid medium: add ethylene glycol 10mL, orange peel powder 2g to 100mL water, (NH 4 ) 2 SO 4 1g, bran 1g, urea 0.05g, CaCl 2 0.01g, adjust the pH value to 5.5;

[0028] (3) Take 50 mL of the liquid medium in step (2) and inoculate it with Aspergillus niger (Asp. 3 a spore;

[0029] (4) Get 250 mg of the seabuckthorn leaf extract powder that step (1) obtains and join in the liquid medium inoculated with Aspergillus niger in step (3), then place it at 30° C., u...

Embodiment 3

[0033] (1) Preparation of seabuckthorn leaf extract powder containing flavonoids: extract seabuckthorn leaves by shaking 80% methanol aqueous solution for 8 hours at 45° C., filter with suction, repeat three times, and combine the extracts. Then, the extract was freeze-dried to obtain the flavonoid compound powder of seabuckthorn leaves, the flavonoid compound content in the powder was 12%, and the total flavonoid content of seabuckthorn was 3.1%;

[0034] (2) Preparation of liquid medium: add ethylene glycol 20mL, orange peel powder 4g to 100mL water, (NH 4 ) 2 SO 4 3g, bran 3g, urea 0.1g, CaCl 2 0.02g, adjust the pH value to 5.2;

[0035] (3) Take 50 mL of the liquid medium in step (2) and inoculate it with Aspergillus niger (Asp. nigercmcc3.316, purchased from China General Microorganism Culture Collection Center (CGMCC)), and inoculate 300 spores per milliliter of medium;

[0036] (4) Get 350 mg of seabuckthorn leaf extract powder that step (1) obtains and join in th...

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Abstract

The invention discloses a preparation method of flavone genins of seabuckthorn leaves, belonging to the field of the preparation method of the flavone genins. In the method, flavonoids compounds are extracted from the seabuckthorn leaves and then are added into a fluid nutrient medium inoculated with Aspergillus niger, wherein the fluid nutrient medium comprises 10-20mL of ethylene glycol, 2-4g of orange peel powder, 1-3g of (NH4)2SO4, 1-3g of bran, 0.05-0.1g of urea and 0.01-0.02g of CaCl2, and the initial pH value is 5.0-5.5; under the condition of 28-42 DEG C, the oscillation and fermentation cultivation can be carried out for 2-10 days; then, under the condition of 40-60 DEG C, the oscillating enzymolysis is carried out for 1-5h; after enzymolysis, the solution rests for centrifugation, the precipitation is dissolved by using methanol, and methanol solution containing the flavone genins can be obtained after filtration. In the invention, the source of the seabuckthorn leaves is extensive, and the biotransformation that the microorganism bacterial strain has the effect on the flavone glucoside in the raw material, therefore, the content of the flavone genins can be increased, and the contents of the three main genins of quercetin, rhizoma kaempferiae and isorhamnetin in the seabuckthorn leaves can be obviously increased.

Description

technical field [0001] The invention relates to a production method of flavonoid aglycone, in particular to a production method of seabuckthorn leaf flavone aglycone, that is, a method for obtaining flavone aglycone from seabuckthorn leaves by using microbial fermentation technology. Background technique [0002] In recent years, many in vivo and in vitro experiments have shown that flavonoids have many biological functions, including anti-inflammatory, anti-allergic, anti-cancer, anti-hypertensive, anti-arthritis and antibacterial activities, reduce chronic diseases such as cardiovascular diseases, and treat skin disease, prevent blood vessel aggregation, anti-allergy, anti-tumor, etc. Seabuckthorn leaves are rich in flavonoids, the content of which is much higher than that in fruits, and the low oil content of leaves also makes it a high-quality source of flavonoids; on the other hand, seabuckthorn leaves have a wide range of sources and have never been fully utilized . ...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12R1/685
Inventor 程永强李刚殷丽君冯淑环李里特
Owner CHINA AGRI UNIV
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